We narrowed to 813 results for: TIM;

... for viewing how gene expression changes through time or after genetic manipulation. The technique involves...

... interact with the bait at a particular point in time, i.e. when the bait is interacting with a particular...

...experiments that require high photostability, such as time-lapse imaging or with high light power detection...

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...

...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...

...not be physically present at your poster at all times, so other participants can identify you. You may...

...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...

...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...

... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...

...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...

...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...

...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...

...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

...contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation is the post-...

...protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to...

...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...

... Editing by John Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts...

... maintaining high brightness and fast maturation time (Figure 1). See the paper for more details on small...

...of course, only hypothetical at this point. More time and energy must be devoted to studying Cas3 and ...