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We narrowed to 867 results for: TOR

Showing: 761 - 780 of 867 results

  1. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...? In this post we will review some of the main factors that affect isotype choice. Target and application...when choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

  7. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...

  8. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen species, ...plasmids that liberate the targeting region from the vector. Alternatively, PCR-generated or commercially synthesized...

  13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend targeting...

  14. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

  15. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... a certain region or calculate a colocalization factor to assess the spatial relationship between the ...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...

  16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...further lower fidelity, like changing the metal cofactor and the dNTP ratio. The resulting random errors...Addgene blog All Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing...

  17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...

  19. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et al., 2014...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...expected location of the target is an important factor in deciding which protocol is likely to work, we...more permanent knockdown, consider using shRNA vectors instead. With both siRNA and shRNA, it can be difficult...
Showing: 761 - 780 of 867 results