We narrowed to 868 results for: TOR

...deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...cells with Nanoblades Primary cells are often refractory to gene delivery methods, but the researchers...

...the experiments performed by our thousands of depositors. That means their original articles are your ...searches scientific articles, patents, and even some doctoral dissertations. Streamline the question asking...

...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...

...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

...deliver in low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al....

...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...

...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

...break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen species, ...plasmids that liberate the targeting region from the vector. Alternatively, PCR-generated or commercially synthesized...

...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...

... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...

... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend targeting...

... a certain region or calculate a colocalization factor to assess the spatial relationship between the ...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...

...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....

...further lower fidelity, like changing the metal cofactor and the dNTP ratio. The resulting random errors...Addgene blog All Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing...

...PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...

...researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et al., 2014...conformational changes that make Cas9 such a valuable laboratory tool are still an area of active research. New...