We narrowed to 811 results for: cha

1. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

2. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...pUC19 Let’s begin at the beginning. The well-characterized bacterial lac operon contains a gene called ...

3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...different biological systems. Although Xie et. al characterized the system in plants, PTGs will likely work ...

4. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...

5. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...

6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ... On the other hand, slow growth can also cause changes in plasmid copy number. During the shift from exponential...

7. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...26814968. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

8. To Each HIS Own

   Type

   Blog Post

   Published

   March 2, 2023, 2:15 p.m.

   ...small size of the polyhistidine tag minimizes the chance of the tag disrupting normal protein function, ...

9. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ...In 2016, I was put in charge of the biggest project I’d seen in my already long tenure at Addgene. I ...

10. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...the previous reactions. This will increase your chances to have a successful and ultimately faster cloning...

11. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...29608282. PubMed Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad...

12. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

13. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary...

14. Click Biology: How to Click and Play to Explore Biology

    Type

    Blog Post

    Published

    Oct. 21, 2025, 1:15 p.m.

    ...from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ... Michael G. Lemieux...

16. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    Published

    July 21, 2016, 2:30 p.m.

    ...promotes gender equality in careers through structural change in research organizations. “Fix the Knowledge” ...

17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...has been successfully applied to functionally characterize combinatorial gene knockouts generated via multiplexed...

18. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

19. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ...Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure...

20. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...