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...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...

...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

...on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes identified...Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement...

...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...

...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...

...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...

...guide. Print out the guide and use it for quick reference when you're designing your next virus experiment...

...support use of their antibody tools. Addgene also offers a growing collection of educational resources on...

...viral vectors and their elements; resources on different types of vectors, capsids, and tools; and helpful...

... Jennifer Tsang...

...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene...