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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 878 results for: ica

Showing: 761 - 780 of 878 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...is simple -  no restriction, ligation or gel purification steps are required! Multiple fragment cloning...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two ...thus marking cells that have undergone genomic modification. In this system, Cas9 must be supplied on a ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...

  3. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

  5. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...

  6. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  7. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...open platform for scientists using AAV to share practical information about their experiences. It will contain...important details that you won’t find in the final publication and we have already collected over 100 entries...

  8. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Rob Hurt, whose expertise and suggestions were critical to the development and writing of two blog posts...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

  9. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...

  10. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...Videos page! Videos are a powerful tool for communicating scientific knowledge. On this new page, we have...  Our first video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our...

  11. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... Jessica Welch...

  12. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ... specificities, from target-specific ones to application-specific ones to anything goes. We've shared ...repositories and search engines Website Targets Application Purpose Notes Antibodypedia Any Any...

  13. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical systems that cannot...

  14. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...

  15. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid...creative hijinks last year. They showed us their dedication to great customer support by dressing up as our...

  16. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...skipping the preparation steps of production, purification, and titering. Our newest viral vector service...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...

  17. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...serotype, species, or promoter) can save you significant time and money. For instance, this report shows...tropism* (min. 300 dpi, PNG format) At least two replicates of your experiment* Functional data, if the report...

  19. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ... any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the...considerations  If your insert contains a mutation or modification, this should be included in the plasmid name...

  20. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular...
Showing: 761 - 780 of 878 results