We narrowed to 860 results for: ple

...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

... committed to understanding some of the basic principles of life and health and works to advance foundational...enhancer sequence, then interact with mediator complexes and the RNA polymerase at the promoter region ...

...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors...

...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

...executing, and troubleshooting your experiments. For example, if you’re very new to antibodies as research tools...

...  Multiple Authors...

...sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng...

... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

...’s Academic and career goals. Career advice for people going into academia: follow your passion and pay...

...biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 ...