We narrowed to 792 results for: por
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TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....community, will soon expand to include several other important pathways. We invite feedback and contributions...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Researchers from a variety of institutions recently reported their inability to recapitulate the results of... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... let us know! Contact our scientific technical support at [email protected] and let us help. Check back... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m.... enough for efficient library performance, an important criterion for probing a limited number of cells... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w Zou... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m.... for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with the selective... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....The GPI-Linked Protein LY6A Drives AAV-PHP. B Transport across the Blood-Brain Barrier." Molecular Therapy... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....isoacceptors induced by amino‐acid starvation." EMBO reports 6.2 (2005): 151-157. PubMed PMID: 15678157. PubMed... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m.... Screens Before diving into the results, it’s important to note that in Morgens et al., guides were considered... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m.... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....Hu et al., 2018). In 2020, the Kleinstiver lab reported the development of near-PAMless Cas9 variants.... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....these reagents in both plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free...