Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 790 results for: Cre

Showing: 31 - 40 of 790 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template...studying the CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...using this library to infect an animal expressing Cre in a cell type of interest, a scientist can isolate...28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread...rounds of infection and screening by PCR enrich for capsid variants with increased ability to infect the...to reach the brain. Delivering the CREATE AAV library via IV screens for capsids that are able to cross...that determines tropism) have been developed that increase transduction efficiency, none allow for simple...

  2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...means to create barcoded gRNA libraries that can be used to combinatorially modify the genome, screen for ...revolutionized how we decode the genome by making it easy to create specific genetic perturbations. The ease with which... off-target activities have proven themselves incredibly useful for the efficient generation of individual...multiplexed CRISPR systems for high-throughput screening are vastly useful for mapping the combinatorial.... Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer...Hiroko, et al. "Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide...PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-...

  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...advantage of these discoveries to create a new system for screening protein-protein interactions. In later...identifying prey proteins after screening. It is also possible to screen libraries of bait and prey against...single-pass two-hybrid screening (6). See the Tips section for more on how to increase your rate of interactions...interactions when screening! Tips for two-hybrid screening Minimizing false positives: Run replicates of ...cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell, including...protein interact and enable intein splicing (5). Screening with yeast two hybrid systems While initial studies...use many preys with a single bait in Y2H-based screens. In this way, proteins that interact with a molecule...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent... pairs of specific sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise...The two recombination sites are both recognized by Cre, but they are comprised of different sequences (loxp...lox2272) and can not recombine with each other. When Cre is introduced to the system it binds one pair of ...sandwiched between 2 loxp sites and is excised by Cre (Figure 1). This results in one loxp site and one...of this spurious expression?   Figure 1.  Cre-recombinase binds to either pair of lox sites, let's...to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify the amount of...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...DIO. Lox sites are recognized by the recombinase Cre. Cre first flips the sequence between the first pair...them.  Lox sites Lox sites, in combination with the Cre recombinase, are used to spatially and temporally...flipped transgene. By restricting the availability of Cre, lox sites lets researchers control the spatial and... activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus...which aids in nuclear export of mRNA and helps increase the expression of your transgene. The WPRE is ...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... your selection, then screen Why add a screen to your cloning strategy? A screen will help you more easily...a look at a few screening methods. Blue-white screen A widely used method of screening is the blue-white...In many cases, you will need a screening strategy as well. In a screen, you aren’t killing off a portion...example on a plasmid, creates a functional 𝛽-galactosidase. Blue-white screens rely on vectors where ...blue-white screening blog post for more information.   Figure 1: Result of a blue-white screen. Image...game. By using a variety of screening and selection techniques, you can increase the chances of finding the...that contain your desired insert? This is where a  screen comes in. Let’s take a look at All_in_one_CRISPR...

  7. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... work more creative. An example could be making lab meetings more interactive and creating space for discussion...tools for creative interaction During Zoom meetings, different tools can be used to promote creative interaction...make the screen-sharing experience more interactive. The button is on the right of the Share Screen control...this is an underrated ritual that really drives creativity and novelty in scientific research and in any...Virtual lab meeting? No problem! You can still creatively collaborate and brainstorm! Image from Joseph...control panel on the top of the screen and every participant get to annotate things as they come up in their...their minds. The final screen can also be saved and exported as a picture. I believe that showing something...

  8. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ... vivo screens in naïve T cells. More broadly, this system can be used to perform in vivo screens in other...power of in vivo screens. Figure 1: Using FITS to perform in vivo CRISPR screens to assess T cell...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...vivo. Over the past five years, in vivo CRISPR screens have been used to interrogate T cells, an important...complex and physiological microenvironments. These screens have primarily been performed in activated T cells...naïve T cells. The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024...

  9. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening in Ghana....cervical cancer screening for a limited group of Ghanaian women by including PCR screening for HR HPV alongside...as positive controls in her PCR screens. She hopes that these screens will both identify individuals who...to be at high risk by these screens can then undergo more routine screening allowing for early cervical...positive controls in PCR-based cervical cancer screens. This third grant represents the continuation of...back in June, Gladys will be using plasmids in creative ways that we wouldn’t necessarily have expected...tests are commonly used in Ghana, there is little screening for DNA markers like the presence of high risk...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...payment for working more than 40 hours per week will increase from $23,660 to $47,476 per year, under updates...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...made by multiple organizations across academia). Increased postdoc wages, overtime tracking? What are institutions...should therefore rise from $43,692 to $47,484, an increase of 8.7%. However, it is hard to gauge exactly ...negotiated a raise from $43,692 to $48,216, an increase of 10.4%. The Icahn School of Medicine at Mount...
Showing: 31 - 40 of 790 results