...70% of human genes have a counterpart in zebrafish. Understanding the function of these genes in fish will...unknown functions for genes, and lays the groundwork for understanding the gene networks that regulate...zebrafish-specific gene expression databases that provide information about when and where genes are active in...investigations. Genetic resources for zebrafish A common approach to unravel the gene networks that drive...model organism is its amenability to genetic manipulation. Tools for gene manipulation together with information...Additional mutagenesis projects have since been initiated and today thousands of mutations in genes affecting...other resources. Targeted gene deactivation tools for zebrafish Random mutagenesis screens provided a major...

...Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous ... worth codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression...ThermoFischers GeneArt Gene Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz Codon Optimization...Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes, so check out our inventory... for a molecular biologist who wants to express genes from one organism in another? Let’s take a look ...correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010...usage frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created...

...among other things) gene delivery, generating pluripotent stem cells, and developing gene therapies for monogenic...and lentiviral vectors that are used for gene regulation, gene of interest expression, knockout, and screening...Experimental uses include: Cre-dependent gene expression, gene knockout, and selection marker removal. ...Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest into...split-vector systems’ by separating viral genes and transgene expression across several plasmids. The most... host genome and thereby enable long-term stable gene expression. Gamma-retroviral vectors are derived...diseases. Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever...

... on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA editing...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, ...protein 2 (MECP2) gene, Rett Syndrome is a rare, progressive, neurodevelopmental genetic disorder that affects...of Rett Syndrome research, the role of the MECP2 gene, and highlights some of the latest breakthrough ...painstaking work trying to isolate the causative gene for Rett Syndrome, Huda Zoghbi's lab discovered ...the first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We...global transcriptional regulator of thousands of genes. Studies have suggested roles in transcriptional...

...with the genes in the compacted region and results in a loss of gene expression. In this way, genes in compacted...the corresponding gene products due to the same region, but not the same exact genes, being improperly...? What exactly is epigenetics? Epigenetics is the study of heritable changes in gene expression that do...makes  genes less transcriptionally active. The role of epigenetics in studying disease Epigenetic changes...your genes documentary PBS Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse... change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling...fascinating examples of epigenetics in the form of imprinting. In imprinting, one copy of a gene (either the maternal...

...trying to target every gene in the genome, or a specific class of genes? Addgene currently carries several...within the target gene. CRISPR can also be used to activate or repress target genes without permanently... gRNAs targeting the same gene can be strong evidence that a particular gene is actually important for...2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...which the underlying genetic cause is not entirely known. Identifying which genes are important for a ... experiment is to generate and screen a population of mutant cells to identify genes involved in a particular...Currently, Addgene carries CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2...

...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is under... research CRISPRa for specific gene targets CRISPR activation for genes with known functions has the potential...uses for Cas9 activators is in genetic screening. gRNAs targeting every gene in the human genome for example...multiple gene loci simultaneously, CRISPRa can also be used to map molecular pathways and genetic interactions...promoter of another gene, thereby driving aberrant transcription. That being said, we generally pick guides by...constructs allow for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting...potential to be used in therapeutics, as it can increase gene expression at certain loci to create changes in ...

...developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression in vivo using ultrasound...plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive...by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists are ...through a host organism, the expression of some genes can change depending on the location. Current methods...of microbes rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue...ultrasound. These ARG plasmids contain genes for “gas vesicles,” a gas-filled protein structure that water-bound...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit to ...

...highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...because you optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express...5. Maertens, Barbara, et al. "Gene optimization mechanisms: A multi‐gene study reveals a high success ...getting your genes of interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid...plasmid at Addgene contains a gene that’s been codon optimized for a particular organism, this will sometimes...available from Addgene now have full sequence data, we recommend directly analyzing gene sequences for ...changing conditions - Organisms often need to express genes at different levels under different conditions. ...

...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and ...further customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length...versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants...Scientists have worked to optimize this system for gene transfer, studying the stability of modified Ti plasmids...versatile binary Ti vector for both transient and stable gene expression applications in plants. This smaller,...and many plant scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity.... They also replaced the promoter regulating the gene of interest with a constitutive, robust M24 promoter...