We narrowed to 897 results for: ANT

...Couple that with a condition known to the plant community as ‘plant blindness’ - the tendency for students...resource or you want to introduce Arabidopsis to your class for the first time – we want to hear from you...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established...year. Introducing Arabidopsis Arabidopsis is an important model system for research and is used by scientists... to be more interested in studying animals than plants, and biology teachers’ preference for using animal...

...Plasmid grant this year. These scientists work on projects spanning from the identification of plant compounds... has worked with both animal and plant viruses. Now focusing on plant biology, she coordinates the Network...Cowpea, a knowledge database for this important agricultural plant. Her current research focuses on endophytes...reside within living plants and that produce compounds with various antimicrobial activities (1). She ...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson... to increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect...

...Bernach. Melissa Chiasson: Quantifying the abundance of thousands of protein variants simultaneously  With ...effects of sequence variants on abundance simultaneously called VAMP-seq, for Variant Abundance by Massively...the abundance of a protein variant library is monitored by fusing the variants to eGFP followed by an internal...the target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found ...University of Canterbury where he studies how bacteria interact with each other and plant surfaces. His...individual’s genome. But when they find genetic variants, what does that mean? Previous research shows ...approximately 75% of all protein-coding pathogenic human variants are decreased in protein abundance, possibly due...

...contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR (Acr) proteins potently inhibit...picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a novel...CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly...indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment...vectors (pUC57-L1L4 and pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...Biotechniques 2012. PubMed PMID: 23066669.Hecker A et al, Plant Physiol 2015. PubMed PMID: 25971551. Discovery ...

...Recommended controls for antibody-based experiments Primary antibody specificity Antibody-based assays have ...Microscopy-based antibody experiments should include a primary antibody specificity control, secondary antibody specificity...species as your primary antibody, like if you are using an anti-mouse IgG antibody in mouse tissue. Such... signal from your anti-Mouse IgG2a secondary antibody, then that secondary antibody is reacting non-specifically...Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies 101: Selecting...confirm primary antibody specificity. The gold standard in demonstrating primary antibody specificity is...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be...

...: Use healthy plants: Your plants should show no signs of stress, such as anthocyanin production, or ... more chances to recover a transformant. Use lots of plants: Lots of plants produce lots of seeds. I typically...select for transformants. It’s very important to select multiple T1 plants from each transformation. I like...process itself can affect the growth of resistant plants so you’ll want to know the effect of your insertion...of non-transformants and make finding true transformants more difficult. Don’t leave your plants on selective...dynamics of genes and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable...genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping your plant’s flowers ...

...smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody (HCab...traditional IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of ...different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site for a total... (DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’...their anti-mouse and -rabbit IgG secondary nanobodies toolbox. Nanobodies are like tiny antibodies which...be attached to each antibody. But being big is a double-edged sword. An antibody’s large size creates ...

...10,000 for primary antibodies). You’ll want to run a protein gradient to test the antibody at the recommended...polyclonal or recombinant multiclonal antibodies to increase overall signal from the primary antibody.  Pro tip... Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not... your blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers...issues.   If your antibody information doesn’t contain blocker recommendations, you may want to try different...make up a fresh batch.  Antibodies We always recommend either using an antibody validated for your protein...interest or validating an antibody yourself. But even with a validated antibody, it can still take time ...

...protocol video!  Antibodies The most important part of your western is your antibodies, particularly your...linearity within detection limits (important if you want to quantify your blots). Fluorescent proteins ...inherently “fuzzier” than gels. Quantification If you want to quantify the amounts of protein in your ...specificity Monoclonal antibodies Increased cost Recombinant antibodies Not always available;...your primary antibodies. We always recommend using an antibody validated for your target in your specific...polyclonal and monoclonal antibodies can work well for a western blot. Polyclonal antibodies, which bind to multiple... The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...

...labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary antibody. While...scale production of antibodies. Problems with antibodies Conventional antibodies are a non-renewable...with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen with a standard... cross-react with anti-rodent or -rabbit Detection with an anti-Chicken IgY antibody is approximately ... (DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...Genetic Tagging - Since antibodies are produced in animals instead of via recombinant DNA, they can’t be genetically...below. The HA tag can be stained for with an anti-HA antibody and serves as a backup means of detection ...

...providers have moved to producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the...blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody...? Why Myc? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into those...monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies for ... peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]). They noticed that ...found that the N-terminal half of the antigen differs significantly between human and the other species,...generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new tools for unraveling...

...purple plant pigments found in all other plants, anthocyanins. Figure 1: An adult plant expressing...resistance to an antibiotic. Successful editing of transformants then typically occurs in T0 plants (from callus...an Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants, but the seeds...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed up ...expressing RUBY plant is a vibrant red/magenta while (bottom panel, left) a lower expressing RUBY plant is a pale...transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance...off-target gene editing. Antibiotic selection would only then kill the desired plants! PCR or fluorescent ...

...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing...-023-38582-7. PMID: 37291094.   Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope... a variety of applications. Find anti-DYKDDDDK recombinant antibodies here!... generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which ...library of AAV9 variants (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was ...compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based...you know that we distribute ready-to-use recombinant antibodies as well? Our collection includes a mouse...

... epitope tag antibodies available through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the...you prefer to produce your own antibody or want to swap out the constant domains, the plasmids for the ...what you'll find in this post: Anti-V5 and other new epitope tag antibodies from IPI New tool for structural...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...performs well in many different antibody-based applications.  IPI’s recombinant version is a fusion of mouse...2024 under CC BY license.    Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel...R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency...

...your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant Parts Kit...Kit II and Plant Parts Kit III (Gantner et al., 2018; Chamness et al., 2023). The Plant Parts II kit has...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic.... The MoClo Plant Parts Kit has everything you would expect in a MoClo kit plus the plant-specific parts...it builds upon (its ‘basics’ MoClo plant kit buddy). It is important to understand your experimental needs...2023). For plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work...sb4001504. Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts ...

...purification of recombinant proteins from plants however can be expensive and time consuming as plant tissues ...Bacterial expression systems When one wants to produce vast quantities of protein rapidly and cheaply, a ...without harsh reagents and patience. Plant expression systems Plants provide a cheap and low-tech means ... mass expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco... have taken advantage of the natural secretion of biochemicals and proteins through plant roots. Tagging...Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...technology, plant cells have been used to express a wide range of proteins including antibodies and pharmaceuticals...

...measured by quantifying the concentration of viral genomes (by qPCR or other DNA quantification methods -...methods: Quantitative PCR (qPCR) Digital droplet PCR (ddPCR) Historically, a quantitative DNA hybridization...titer AAV! The point is there are many ways to quantify the composition of a viral vector solution, and... characteristics give us information on three important factors. What can AAV titers tell you? Physical...transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type AAV2 has... of 1:1 (Zeltner et al., 2010). However, for recombinant AAV2, the same study reported a physical-to-infectious...2010), while another reported that only 20% of recombinant AAV2 particles were full, compared to 50% of ...

...extensions and, most importantly, any connections the neurons make to other distant brain areas. It is ...axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport pathways...J.T., Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits...implications their signaling is likely to have). Anterograde tracing outlines neurons from their cell bodies...pathways in neurons. Anterograde transport is typically used for the trafficking of organelles, such as ...retrograde transport relies on dynein, while anterograde transport relies on kinesin. Different rates ...of speed for various forms of retrograde and anterograde transport suggest that there are several parallel...

...individual variants found in tumors are exceedingly rare. As a result, how most of these “variants of unknown... available collection of mutant cDNA clones corresponding to cancer variants found in genome sequencing...include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived from analysis of ...Cancer Mutant Collection) or “pan-cancer” analyses across many cancer types (Pan Cancer Mutant Collection...cancer clinical sequencing report includes rare variants that haven’t been characterized? Some have developed...approach this challenge, using attributes of cancer variants such as evolutionary conservation, or predicted...hope researchers can use to characterize these variants in a high-throughput manner and therefore push...

...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to...scatter effect is also an important consideration when preparing the implant itself, which brings me to...polished to significant smoothness to prevent drastic loss of efficiency between patch cable and implant. A steady...optogenetic implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not...opsin through a small piece of fiber optic cable implanted into the animal’s skull (right). The fiber optic...skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic patch cable is...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...

...principal advantage of epitope tags for IHC is the availability of reliable primary antibodies for their...particularly when antibodies to the POI are non-specific, cross-react with other antibodies, or are unavailable...FPs with facile antibody recognition of epitope tags. Thus they have created antigenic protein tags called...their direct fluorescent signals or using anti-FP antibodies. GFP emits light in only a single channel...prone to aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor...their multiple, highly specific antibody binding sites and primary antibody-based detection, smFPs create...neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been widely ...

...with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] -... New recombinant antibodies targeting Glypicans By Ashley Waldron Glypicans are important modulators...view from the antibody pages or in the Addgene Antibody Data Hub. Perhaps these antibodies are just the...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR ...By Mike LacyGlutamate is one of the most important and abundant neurotransmitters, but it is challenging...large library of iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation...with 96 h lentiviral supernatant and selected with puromycin. Puromycin-resistant cells were fixed and...

...two antibodies against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP ...doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By Ashley...Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human genes and are recommended...the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5...-A5] recombinant antibody in human postmortem brain samples. Results show positive staining in tissues... Find more neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry...months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...

... Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl tRNA synthetases...Validated recombinant antibody toolbox for aminoacyl tRNA synthetases By Kate Harten Recombinant antibodies...Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based...for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes...binding, but impact recognition by specific anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral...these processes. To create the antibody toolbox, they screened antibody candidates using a synthetic human...whether the generated antibodies could bind these aaRSs. They found the antibodies were able to immunoprecipitate...

...s Neuroscience Antibody Collection to find a recombinant anti-GFAP [N206A/8R] antibody shared by James...well as antibodies for identifying other cell types in the nervous system!  Find recombinant anti-GFAP [...editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM ...recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded...TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system...N206A/8R] antibody here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The...

...Availability Antibodies 101: Introduction to Antibodies Resources on Addgene.org Find recombinant antibodies...recover good antibody binding by tweaking those parameters, but in some cases your antibody may just not... generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it... the Addgene blog: Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Epitope Availability...antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene  ...your samples in a given state or point in time. You want to halt degradative processes while maintaining ...epitope of some targets, preventing your primary antibody from binding (Fig.1). The level of cross-linking...

...expressing plasmid to generate a population of mutant cells. The mutant cells were treated with WNV at a dose ...editing and mutant library generation in several biotechnologically and clinically relevant bacterial species...and CHIP-seq), genome-wide identification and quantification of enhancer activity have remained challenging...sufficient to kill all control cells and surviving “mutant” colonies were deep sequenced to identify the gRNAs...Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts to do so have...gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts...vivo expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall, et al...

...research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] and Anti-SARS-CoV-2 Nucleocapsid...Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated and characterized... for details on the original antibodies. Find Anti-SARS-CoV-2 Antibodies here! Terry, J.S., et al. (2021...38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use recombinant antibodies...were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies #211756 (left panel) or #211757...months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our...pooled CRISPR screens Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector...

...context of significant clinical uncertainties and a rapidly evolving healthcare space. The consultants who enjoy...parts of the job. Is consulting meant for PhDs? Healthcare consultants are usually trained in the life...the things that pleasantly surprised me in my first few months as a healthcare consultant was how much my...great fit for former healthcare consultants. I have even seen consultants go back to research and bench ... I’ll share what I’ve learned as a healthcare consultant and give you an idea of what a career in this...skills you’ll need to do well as a healthcare consultant and what you could do to break into the field.... What is healthcare consulting? Healthcare consultants advise pharmaceutical companies, biotech start-ups...

...Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A...34385696; PMCID: PMC9015817.   Meet anti-GFP [N86/8R]! by: Ashley Waldron Anti-GFP [N86/38.1R] was among the...our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and we ...second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant form by...reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform...section on the Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R... toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling...