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...path. Because there is no demand for a favor, the person you ask will be more likely to say yes and more... Informational Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at... your interviewee? It is always best to meet in person to develop relationships, but if it is not possible.... Keep in mind that this is a good time to ask personal questions about salary, opportunities for advancement...advancement, challenges and disappointments. These more personal questions can elicit the most valuable insights...

...secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery...created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly...Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP for protein... PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible...from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV...

... should I try research? Personally, I have always been a pretty curious person. I like to ask questions...anyone else in the world knows it—to be the first person to make a discovery. I can’t tell anyone else what...place to begin. Here’s my advice: Reflect on your personal interests While it’s perfectly fine to be unsure... also makes for an experience more tailored to personal interests. Talk to people, ask questions, and ...First-Generation College Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/...

...DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved the DNA double helix...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...movies. Rampage (2018), starring Dwayne “The Rock” Johnson, calls out CRISPR as the genetic engineering experiment...Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable...:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure...

...and Hoess, 1984; Van Duyne, 2001; Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors ...PubMed PMID: 11340053. Wick, Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and...Nature 351.6326 (1991): 489. Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic...PMCID: PMC5111696. Towne, Chris, and Kimberly R. Thompson. "Overview on research and clinical applications...

...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... classification This inquiry started when Neil Thompson, a research scientist at MIT’s Computer Science...query coverage) that could be determined through comparisons with a reference sequence database. Through a...Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene synthesis allows biologists to source genes...

...proteins without changing excitation wavelength (Salomonnson et al. 2012). They can also be excited efficiently...emission intensities from fluorescent proteins (Salomonnson et al. 2012). Finally, it is worthwhile to test...18718768. PubMed Central PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during...18261937. PubMed Central PMCID: PMC2390753. 4. Egen, Jackson G., et al. "Intravital imaging reveals limited ...21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral...Wolfgang Weninger. "Dendritic cell behaviour in vivo: lessons learned from intravital two-photon microscopy."...

...favourable ratings to the abstracts that affirmed their personal beliefs even though all three texts were otherwise...were exhibiting a phenomenon called “motivated reasoning,” the catchall term for a range of neat little...little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More specifically, they were engaging...sources of information they don’t like, looking for reasons to dismiss them. There’s evidence to show that ...nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin, 108(3), 480. More resources...

...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days...student engagement and joy, rather than a perfect lesson plan or outstanding test scores.  As a result, ...are provided with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing...investigate together. Teachers decide which BioBuilder lessons to teach, and how — some choose to run full semester-long...semester-long classes, some pick and choose a few lessons to include each year; others use BioBuilder as ...

...essential for the structure of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess...your favorite biological phenomenon, there’s a reasonable good chance that Addgene can help. Though it ...Fox Elaine Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander Stark Bas..., especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin...

..., and substitutions - at the same efficiency as Gibson Assembly and GeneArt cloning - but at a much lower...digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC can all be ...When REPLACR-mutagenesis goes head-to-head with Gibson assembly and GeneArt cloning, the results are favorable...hands!     REPLACR-Mutagenesis Megaprimer PCR Gibson Assembly GeneArt Cloning Types of Mutations Insertions...Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis with other common methods...

...Twitter, LinkedIn and Facebook to see their online personas. Some have online coaching tools that can be very...remotely (by phone, Skype or Google Hangout) but in-person coaching is nice if you can get it.  Because I ...this offers the option of matching with the best person in the group for your needs. Beth Schachter of ... Michelle Capes at Adeptify Me. While based in Madison, Wisconsin she also works remotely and has experience...support for issues of diversity in science.  A great person to contact is Twitter’s @DrQualls. Marquita Qualls...

...triggering immunotoxicity. PM577a: Prime editing for Wilson's disease Maria Collier (Prime Medicine) presented...liver to correct the ATP7B p.H1069Q mutation in Wilson’s disease. Results showed >80% precise correction...Striepen, J., Maciejowski, J., Green, A. M., & Buisson, R. (2025). Mechanism of DNA replication fork breakage...N. (2025). Employing RNA editing to engineer personalized tumor-specific neoantigens (editopes). bioRxiv..., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna...Editing The Advances Behind The World's First Personalized CRISPR Treatment Resources on addgene.org ...

...genetic tools. Mobile-CRISPRi from the labs of Jason Peters, Oren Rosenberg, and Carol Gross provides...Mobile-CRISPRi is introduced using the bacterial Tn7 transposon system. This system has two parts: a plasmid ...Introducing Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid...methods 2.6 (2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative...4.2 (2019): 212. PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse...

...Kostick, K., Torgerson, L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram... rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https...00981-0 Wright, J. F., Le, T., Prado, J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce... Jason Nasse...

... to put to rest a common misconception. I find personal bias often unintentionally plays a big role in...varied in how they prefer to consume information. Personally, I would much rather read the instructions than... accessible via web searches; likewise, most in-person events and venues will have a solid understanding...Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low (except for ...  Rachel Leeson...

... Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...This post was contributed by Jake Watson and Javier García-Nafría from the MRC Laboratory of Molecular...receptor function.     Follow them on Twitter @jakefwatson and @JGarciaNafria.  References 1. Jones, Douglas...PMCID: PMC331480. 3. García-Nafría, Javier, Jake F. Watson, and Ingo H. Greger. "IVA cloning: a single-tube...

...libraries that do just that (Doench et al., 2016, Sanson et al., 2018). These libraries utilize optimized...additional transcription factors to the targeted region (Sanson et al., 2018) . This tracrRNA was combined with...selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese library ... more hits than the SAM library approach in a comparison study screening for vemurafenib resistance in...: 25494202. PubMed Centra PMCID: MC4420636.  5. Sanson, Kendall R., et al. "Up, down, and out: next generation...

...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...your community’s schools, here are a few tips and lessons I’ve picked up from my recent experiences. STEM...history helped demonstrate that a) I was a real person who used to be a middle schooler himself and b)...back and trying again next year. There are many reasons why you might want to reach out to your school ...tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements: I...

...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets... C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base ...doi.org/10.1038/nbt.3803 Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti... Bohnuud, T., Fernandez, T. P., Gehrke, J. M., Grayson, P., Lee, K. D., Ortega, M. A., Sawyer, C., Schwaegerle..., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna...Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt, J....

...comes to genome editing, CRISPR is a name that resonates with nearly every biologist, academic, and researcher...also need a short sequence motif called a TAM (transposon-associated motif), which is positioned like Cas12a...parts: a structural scaffold derived from the transposon's right-end element, and a 20-nucleotide (nt) ...genome editing in monocot and dicot plants using a Transposon-Associated TNPB system. In Springer protocols...). A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome editing...

...service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop a reliable...available. This prioritization happens for good reasons. We began offering our viral vector service because...our Viral Vectors 101 eBook and blog posts. The lessons learned from this process have shaped and supported...fnana.2019.00093 McDade, J. R., Waxmonsky, N. C., Swanson, L. E., & Fan, M. (2016). Practical Considerations... Rachel Leeson...

...CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global transcriptional ...rescue the neurological defects of Rett syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett...Supplement/S51.002.abstract Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020)... . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J...398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 ...

...organisms have different codon usage biases? The reasons for varied codon preferences among organisms aren...aren’t completely understood, but some possible reasons include: Metabolic pressures - it takes cellular...recognize codons found in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression...15678157. PubMed Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence... 2265611. PubMed Central PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy Minshull...

.... 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances in fluorescent protein... structure resembles images of the deity (see comparison image above). The church of the Flying Spaghetti...schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University...New York. pp 719. 3.Rizzo, Mark A., Michael W. Davidson, and David W. Piston. "Fluorescent protein tracking...

...know your collaborators from other institutions personally on conference calls and make sure to maintain...collaborating with people outside my lab’s department, I personally felt that I had to overcome some hesitation to...the hard part: maintaining these relationships. Personally, I find this to be the most challenging and time-intensive...maintain a relationship - many of them involve personal meetings and common interest so naturally this...sharing. LinkedIn or ResearchGate allow you to send personal updates as messages and keep you up-to-date on...

...advice, open doors for you, and teach you valuable lessons along the way. We think it’s so important that ... technician, or a graduate student. This is the person you report or talk to on a daily basis who will...scared to ask for some! You can email or ask in-person “Are there any papers you think would be useful...these things will help you get more out of the in-person training component. Documenting during training...take on trainees one day when you are the senior personnel! Happy training! Resources Resources on the Addgene...

...are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013), which has been shown ...2011): 8604-8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL,... therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput droplet digital...

...Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner...of TSS for best results but up to 400 bp works reasonably well.  Best of luck on your experiments!  Many...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

...10.3390/antib8020028  Bird R, Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan... (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv.   What...doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny J, Margolies... made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular Biology 275:245–253 . https...