We narrowed to 662 results for: Clu

...inside the body. So we screened gas vesicles gene clusters from a diverse set of gas vesicle-expressing species...right: colony whiteness on inducer plates, white inclusions in cells with GVs via phase contrast microscopy...this was the Anabaena flos-aquae gas vesicle gene cluster because of its small size and its ability to produce...a time-intensive endeavor. That is why we also included an analogous set of transient constitutively-expressed...

...made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 kb) from Streptococcus... approximately 1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000...in mouse liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split...

... hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular..., PhD, and Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular...measurable cell toxicity – using Cas7-11. This included tests done in cell lines known to be more sensitive... — the ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR...

...an interview next week and it includes a chalk talk. What should I include in my chalk talk?" So what exactly...proposed approach to resolve the knowledge gap? This includes the overview and the individual aims. Describe...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

...domain - VP64.  Later generations of activators include the SunTag system which facilitates recruitment...Addgene’s website. Fig. 3: Cas9 repressors include dCas9 alone and a dCas9-KRAB fusion, both targeted...targeted to the promoter region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors...expression in different species and cell types including (but not limited to) bacteria, yeast, plants, ...

...but some examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place...resolve these issues is with electrophysiology. Concluding thoughts and moving beyond the mouse While it...applicable to the rat [6]. Technical differences include the ferrule implants and patch cables. One huge... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

...Several factors contribute to the popularity of GFP including (i) fast and complete maturation to functional...endogenous fluorescent co-factors can be used. Examples include the LOV domains that bind flavin (Buckley et al... conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP (pKa = 3.1) and... or media. These sources of ‘autofluorescence’ include riboflavin in the medium or flavin containing proteins...

...known as steric hindrance. For instance, GFP may occlude catalytic sites or obstruct binding sites or motifs...proteins that behave as monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), ...motif, which is needed for Golgi localization, is occluded and therefore this protein is mislocalized. The...that the fusion behaves well. But this is not conclusive evidence that the fusion operates in the same...

...works. Advances in Cre-lox recombination have included inducible Cre, such as the tamoxifen-responsive...mediate recombination.) The deletions are mutually exclusive, as either deletion removes one of the other loxP...immediately following the promoter. The STOP cassette includes a mutant YFP that does not fluoresce, but can ...More variants have been created by other labs, including R26R-Confetti described in Hugo J. Snippert, et...

...in a variety of neuronal imaging applications, including confocal and widefield imaging, two-photon microscopy...several endogenous or pathological condensates, including oncogenic transcription, proliferation of leukemia...to the adipose cells utilizing glucose to the exclusion and detriment of the cancer cells.  Figure...al. (2025) under CC BY license. The paper includes other great feats of materials engineering as ...

...organizations in a variety of science policy roles including fellowships, volunteer positions, and part-time... with a large set of other societal pressures including economic, cultural, and political. Science policy...science blogs. Other avenues for skill development include leadership of extracurricular organizations, working...opportunities. NSPN maintains a great resource guide including conferences, societies, and outreach groups. Grab...

... molecule at all, leading to inconclusive results, erroneous conclusions, and wasted time. In fact, many...antibody-dependent approach. Antibody-independent methods may include analysis of transcriptomic or proteomic data from...specifically to the expected protein. Common approaches include using RNAi to knock-down expression of your target...

...for CRISPR genotyping. The most common options include mismatch cleavage assays, such as Surveyor™, T7E1...information profile regarding putative off-target sites including both the edit rate and the repair product sequence...algorithms do not take into account other elements, including those relating to DNA structure (e.g. epigenetic...options exist for unbiased off-target detection, including Digenome-seq (Kim et al. 2015) for in vitro analysis...

... effectors and are the only CRISPR type that exclusively targets RNA. Cas13 has become a natural successor...CRISPR will likely depend on a variety of factors, including your goal, experience, hypothesis, and even the...keep increasing, as efforts using large-scale clustering and discovery algorithms find new candidates ...of rare CRISPR-Cas systems with deep terascale clustering. Science, 382(6673). https://doi.org/10.1126/...

...organism to make the complicated transition from a clump of cells to a fully differentiated body. In contrast...monitor cell interactions in diverse tissues, including lymph nodes, brain, liver, gut and skin. Two-...able to help answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the...probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange...

... Figure 1: Labeled diagram of an antibody including Fc, Fab, heavy chain, light chain, constant region...antibodies for experiments in a variety of fields including neuroscience and immunology. They can be used ...analyzed using techniques such as flow cytometry. Conclusion Whew! Now you know that antibodies can be used...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, ...

...main experimental avenues (that are not mutually exclusive) commonly used in optogenetics: 1) electrophysiology....  Of course, proper controls are required and include, for example, confirmation of opsin expression ... brief, some standard opsins (see table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

... this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find five photos this... the blog. Below each photo, the pet gives you a clue to help you find the next picture. If you find the...

...sneak in from almost any step of the process and include things like genomic DNA, RNA, proteins, salts, ...sustainable practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification... kits from a variety of manufacturers. It also includes a rough comparison of list price between these...Yeast, BAC, transfection-grade While we’ve included a sample of some commonly used kits, please note...

...for plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!   ...destination vectors. Destination vector options include humanized wt Cas9, dCas9 (transcriptional repressor... Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA cassette, Csy4-cleavable ...filler sequences. You also have the option of including a previously-designed tyr gRNA, which causes hypopigmentation...

...new edition of the eBook includes the details on advances in CRISPR including: Base editing Prime editing...CRISPR Cheat Sheet here!   CRISPR Cheat Sheet Clustered Regularly Interspaced Short Palindromic Repeats...

...for plasmid. pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This...backbones, visit Vector Database. Step 2: Insert name  Include information about the insert in your plasmid name...contains a mutation or modification, this should be included in the plasmid name. Mutations are generally listed...

...the template. The PE3 system does just this by including an additional sgRNA. Using this sgRNA, the prime... number of unintended prime editing outcomes, including indels. Therefore, they engineered a dominant-...     ✓ ✓ reflects an innovation always included in the indicated tool. ~ reflects an innovation... strategies in our CRISPR guide. Some examples include: TwinPE: two prime editors make complementary ...

...species, antibody species, or whether the results include knockout data.    Figure 2: Search options in ...description of the experiment. If that data was included in a publication, you'll find that information... is easy!  Here at Addgene, we love all data, including negative data, which can help researchers save...

... to a post-academic career. This training was “included” with their tuition, so to speak.  Unfortunately...the development of priorities for pd|hub which include building capacity to identify the most effective...scientists, but it is essential that we integrate inclusive career readiness training in this educational ...

...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

... show resistance to stressful circumstances – including unpredictable loud noises and flashing lights ... of the animals’ skeletal muscles led to another clue: differences in the kynurenine pathway of tryptophan... treatments for a variety of brain disorders, including psychiatric and cognitive conditions as well as...

...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

...submissions via email: [email protected] Entries include accession number, species, and assigned category...profiling for human, mouse, and dog cell lines is included when available, and can be submitted via email...Depending on available information, entries may also include whether a cell line is known to be genetically ...

...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

... by any number of characteristics. Common ones include age range, familiarity with the subject, shared... that by defining a specific audience, they’ll exclude others from accessing their work. But that’s not...science communication work does not explicitly exclude people outside of the original recipients. Instead...

...allow researchers to understand bacterial gene clusters that produce useful natural materials, such as...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

...the mid-20th century, and many iconic papers, including the ones on the structure of the DNA, were not...first couple of sentences - even if written in a clumsy language make it much easier for me to go back ...initial read, adjust my language in the first (and clunky) version of my general comments and adjust them...

...for half of the trip. Preparations ahead of time included contacting YNP staff to arrange permits to visit...frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box to monitor...route that would reach all of the remote areas, including one hot spring requiring an overnight hike in....