We narrowed to 291 results for: Fus

...catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules to repress or activate...consist of the GAL4 or ZIF268 DNA binding domains fused to either the hER or hPR ligand binding domains ...

...I suspected I knew at least one source of the confusion in our meetings. A lot of our LIMS planning involved...can help the team identify possible points of confusion in the future. Improving cross team communication...

... as clear as you can be (there will always be confusion about this though) about the fact that containers...them to their contents might leave you sad and confused when a tricky procedure failed and one of your...

...Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. You ...windows” you’d like the tool to use to identify fusion sequences for either a circular or linear assembly...

...varied as it can have important implications for the fusion protein effectivity), and a fluorescent protein...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible...

...domain. Because ABE7.10 contains a heterodimeric fusion of wild-type TadA and TadA7.10, they designed one...developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA region. ...

...particles are smaller and may be preferable if wider diffusion is required. On the other hand, lentiviral vectors...has to be slow to allow the viral particles to diffuse away from the injection site, and also to minimize...

...then be fused to effector domains such as transcriptional activators or repressors. These fusion proteins...

...(SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct...flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine base editor architecture...

...construct, 3 copies of the fluorescent protein were fused in-frame to a tethering partner, which directed ...enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek and Neveu, 2015...

...envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system...in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but...

...swapping and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, ...Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants’, Bioscience...

...across a couple other terms that can cause some confusion. Paraformaldehyde: Many IF protocols call for...crossing cell membranes as they are not able to diffuse across natively. Permeabilization essentially punches...

... 18;127(7):839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene ...Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco...

...prime editing (Molla et al., 2021), which require fusing Cas proteins with additional enzymes or domains...also function as an efficient gene activator when fused to a transcriptional activation domain (Karmakar...

...MoClo nomenclature and structure can be quite confusing for new users, and your cloning success depends...terminator, or other plasmid part? Do you want to fuse your protein to any tags or fluorescent markers?...

...versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of...antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and...

...triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright targeted...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor...

.... A common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain...Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus where...

...video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria for antibiotic resistance... treatment. This is called a Kirby-Bauer disk diffusion test or Kirby-Bauer susceptibility test (Figure...

...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...

...the construct, dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these...epitopes and fluoresce in the same color as the fused XFP can be used to amplify the signal. Imaging endogenous...

...HiFi DNA Assembly (NEB) Cold Fusion Cloning (System Biosciences) In-fusion Cloning (Clontech) Although...

...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...

...collection contains many self-labeling protein fusions as well as empty backbones to clone your own protein...homeostasis maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using...

...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...

...published and documented by OMICtools. It must be confusing for a newcomer to decide on the right software...of ideas is what makes choosing the right tool confusing and it means that there is no one-size-fits-all...

...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...

...libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency. Listen ...

...you have a question about culturing iPS cells? Confused by which system to use for genome editing? Know...

...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

...monoclonal antibody cell line, myeloma cells are fused with activated B cells from the spleen of mice injected...

...substrate for a kinase of interest which is further fused to a bipartite nuclear localization signal (bNLS...

...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...

...might say yes and they might say no but do not confuse networking for a job connection with ongoing relationship...

... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ...

...complete their work rather than overwhelmed or confused about what’s coming next. Goal setting and career...

...Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive...

...hard to find. This made the page look not only confusing, but also made it hard for users to get where ...

...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three...

.... A Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat ...

...amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher onto things, but even better if one could...

... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...

... which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize target...

...chemogenetics relies on chemical activation and the diffusion of drugs throughout the body, the onset of receptor...

...constructs. For the Gγ constructs, the GFP2 tag is fused to the N-terminus of the protein. For the Gα constructs...

...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...

... (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain tissue. They performed optical...

... References Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to...

...that respond to different pH’s and some that are fused to fluorescent proteins. She also showed that R-...