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  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...varied as it can have important implications for the fusion protein effectivity), and a fluorescent protein...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...(SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct...flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine base editor architecture...

  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...construct, 3 copies of the fluorescent protein were fused in-frame to a tethering partner, which directed ...enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek and Neveu, 2015...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system...in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but...

  6. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...across a couple other terms that can cause some confusion. Paraformaldehyde: Many IF protocols call for...crossing cell membranes as they are not able to diffuse across natively. Permeabilization essentially punches...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...swapping and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, ...Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants’, Bioscience...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... 18;127(7):839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene ...Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria for antibiotic resistance... treatment. This is called a Kirby-Bauer disk diffusion test or Kirby-Bauer susceptibility test (Figure...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright targeted...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of...antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and...

  12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    .... A common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain...Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus where...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...

  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...

  21. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

  24. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...

  26. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ...

  28. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers such as TET1 with inactive...

  30. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three...

  34. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...

  35. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize target...

  36. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...constructs. For the Gγ constructs, the GFP2 tag is fused to the N-terminus of the protein. For the Gα constructs...

  38. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...that respond to different pH’s and some that are fused to fluorescent proteins. She also showed that R-...

  39. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ....” You can see how you could end up in a very confusing situation rather quickly!  The question   The...

  40. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... References Terpe, K., Overview of tag protein fusions: from molecular and biochemical fundamentals to...

  42. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Scanziani New viral prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...

  43. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer...

  49. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ....  Why the MTA?  The MTA process can be a bit confusing, and it’s certainly frustrating to find out your...
Showing: 151 - 200 of 282 results