We narrowed to 528 results for: Set

...are ready to begin the screening process. Want to set yourself up for a screening success? Before you start...look for off-target changes. This method requires a set of control cells to compare the sequencing reads ...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...-day experiment in the lab. You have two ways to set up the experiment. The first way, it will take you... project (with metrics if possible) and, at the outset of a task, I outline the activities that are needed...

...newly synthesized DNA strand. Each degenerate primer set contains three consecutive randomized bases (NNN)...NNN) at a given codon site and multiple primer sets tile across the protein or region of interest. A low...

...from someone seeing me speak in a non-interview setting. There are many resources on self-promotion (how...of this article will be talks for non-academic settings. Many of these guidelines will apply to any type...

...contamination with RNase leading to degradation that can set you back days. Working with plasmid DNA is much easier...pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple PCR with ...contains humanized Cas9 expression cassette and sgRNA expression cassette. The target sgRNA can be cloned...of validation for DSB mediated EGFP expression cassette reconstitution. When the target sequence was digested...took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA...

...energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely to alter...

...while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists...

...careers. Failure to strengthen the talent pipeline sets the world up for reduced impact of scientists and...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...

...  Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

... recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse...

...endeavor.  Break the process down into steps and set aside time during the week to tackle each one.  Get...

...the biggest hurdles for scientific sharing. So we set out to streamline this process. We take pride in ...

... Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells because...

...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...

...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...

...specifically developed to thrive in laboratory settings. One of its most attractive features is its stability...easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms...

...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...

...Engine for RPA-Cas12a (CASPER). CASPER produced a set of primers and sgRNA that experimentally outperformed... our new team together and our project direction set. We're staying in the environmental space again and...

...machine learning approach, the lab proposed multiple sets of Cas9 mutations that conferred new PAM preferences...mutations produced efficient editors, the Kleinstiver set performed best when paired with the V106W mutation...

...extracted, sectioned, and imaged, with all image sets available at the Allen Institute’s Genetic Tools...Enhancer Collection is a quick guide to a smaller set of tried-and-true winners, containing enhancers with...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...

... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...

...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...

...For each of these 17 pathways, they identified a set of one to three mutant complementary DNAs (cDNAs)...

...that will put you in position to pursue a diverse set of career paths. Check out Joanne's Reddit AMA Here...

...Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested...

...Amy say is the quickest way to get things started? Set up your own Science by the Pint series and organize...

... part of my job, I travel around the country and set up meetings with PIs to discuss the benefits of depositing...

... questions for specific feedback in non-public settings. Ask over coffee.  Have regular 1:1 meetings with...

...think I should talk to?” That way you are always setting up your next opportunity to learn. Additional ...

...Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange automatic email reminders....

...fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part of...may make sense to try multiple CRISPR tools when setting up your experimental system. Let us know about ...

...and Ioannidis 2005) and changing an experimental set-up partway through an experiment in an attempt to...a journal prior to performing the experiment (or set of experiments), the design would be reviewed, and...biological researchers deal with larger and larger datasets and use computer based analysis techniques, an...

... produce a BRET probe for imaging. These authors set out to design a better fluorescent protein that could...could be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...impractical or infeasible for certain experimental setups. The brightness of NLuc has caught the attention...

...laboratories remains limited. Members of the Stanley Qi Lab set out to address this limitation by developing CRISPRkit...inexpensive system for CRISPR experiments in classroom settings without specialized equipment (Collins & Lau et... et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's disease model...

...researchers are eager to apply it in a clinical setting. CRISPR is often used for animal germline modification...to deliver via gene therapy, so researchers have set their sights on another approach. Since shorter forms...

...different regions of the brain. A more locally defined set of neurons can then be targeted with optical stimuli...

...lifecycle. Halloween genes The Halloween genes are a set of cytochrome P450 genes discovered in Drosophila...

...Science in Boston, where the entire Blue Wing was set aside for the event. As guests arrived, some gravitated...

...image courtesy of jorenl/Vecteezy.com Helps set concrete goals and helps you reach your goals Listens...

... to complement our next-generation sequencing, setting new standards for plasmid quality control. Through...

...target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences...

...available to scientists working in a for-profit setting.  In light of the need for basic research during...

...replication experiment that you conducted as part of setting up your project. These data are valuable but remain...

...be useful to you.) With these goals in mind, we set out to update our web resources and plasmid pages...

...Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday evening, I stayed...

...in writing your own blog post about a plasmid or set of plasmids you’ve deposited with us. Don't forget...

...Freshers Fair, all of the societies within a university set up stalls or stands on campus and recruit students...network, find groups with common interests, and settle into university life. The fairs are also an opportunity...

..., cells may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns...

... 1. Keep up with the literature. He recommends setting up a few Pubmed email alerts for new papers with...

... the transparency window of mammalian tissues is set by the properties of hemoglobin and melanin pigments...