... should contain a reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence...et al. Nat Biotech 2003): In the presence of Cre, GFP expression is lost, allowing for rescued expression...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

...hiPSCs  EMX1  67% Mouse BM cells from GFP transgenic mouse  GFP  ~60-65% Human hepatocytes  Myd88  ~...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery...

...plasmids described in this work including the soCoChR-GFP plasmid as well as several derivatives encoding alternative...ShadowG, a novel dark green fluorescent protein (GFP) with an absorption peak of 486 nm and ShadowY, a...CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with...

... FRET tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools...hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre Marillonnet share...and since we are making all the tools for plant research here and validating them, it made the most sense...academic in literature who gave a talk to the women’s research network here. She talked about the advantages ...

...drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes...homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further development of this...efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.  PAM sequence... design of these constructs, allowing novice researchers to quickly and easily start genome editing (Schwartz...donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289...CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805...

... hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance...  After validating this method through monitoring GFP degradation, this protein compartment was used to...targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

...overlap. Figure 4 compares the spectra of enhanced GFP (eGFP) and FITC, the latter being a commonly used fluorophore...to fuse them with a fluorescent reporter, such as GFP or mCherry, through genetic modification. As your... you can also use fluorescent reporters, such as GFP, and fluorophore-conjugated antibodies together in...vector also encodes for eGFP, which serves as a transduction reporter. Note that eGFP is not fused to CD45...-Cy7. Next comes our transduction reporter, eGFP. As eGFP emits fluorescence by itself and does not need...cytometer which channel to use to detect eGFP. As you saw in Figure 4, eGFP strongly overlaps with FITC, hence...two compounds are completely different in nature (eGFP being a protein and FITC an organic molecule), their...

...red light instead of blue to excite a molecule of GFP. Because red light has approximately half the energy...light, two photons of red light are needed to excite GFP compared to only one of blue. Only at the focal point...infinitely small point of light – think of a single GFP molecule – will appear as a fuzzy 200-300 nm spot...of tissue clearing techniques (9) now allows researchers to image tissues that are over a cm3 in size....

...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can...meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid distribution...distribution is booming, but, over the years, many researchers we’ve met with have asked if we can help them share...distribution came up time and time again with many researchers lamenting the viral production process while ... We’re reducing this frustration and getting researchers to the experiments they’re interested in faster...processes of viral production and testing so that researchers can use our educational resources and experience... decide to produce virus on their own.Harvard researcher Connie Cepko has been helping us with AAV testing...

... University with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore...and technologies in life sciences research. The rare disease research community has certainly noticed: ... Institute sponsors a global competition for researchers working on rare diseases, offering them the chance...year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award attracted submissions from ... OuYang, BeHEARD Co-Director in a release. The research teams studying four of those rare diseases will...wrongly diagnosed as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease...in coloring of the hair, skin, and eyes. The researchers found that seven patients diagnosed at their ...

... cassette with an ubiquitous promoter to express GFP in a group of young rats. We found that, in this ...label norepinephrine (NE) expressing neurons and GFP (green) to label neurons transduced by each serotype...acknowledge that negative data is something every researcher experiences and that it often provides incredibly...and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists...regions and cell types. Power in numbers A Pubmed search for “AAV” and “brain” over the past decade gives...., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives on Data Sharing in Deep Brain Stimulation...Data Freedom: The Expansion of Data Sharing in Research Publications...

...antibodies against popular fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have...Human Development (NICHD) in 1986, to enable basic research by banking and distributing monoclonal antibodies...have also created the Monoclonal Antibody Research Institute dedicated to: 1) developing new ways of generating...Data Freedom: The Expansion of Data Sharing in Research Publications ...

...chemistry! The discovery of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly...advances lead to an obvious question: In this age of GFP and its ilk, why should cell biologists talk to chemists...blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry...cell biologist can feel like a college student searching couch cushions for spare change, desperate to ...Lavis is currently a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute. He would...

...tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to.... "Evaluation and comparison of the GUS, LUC and GFP reporter system for gene expression studies in plants...Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production either in the plant...help in screening candidate genes and speeding up research. Certainly, there is still more to transient leaf...your lab, it can become a strong tool for your research. Feel free to add further tips and experiences...

...mouse brains expressing green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later...subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H mice).  However, we recently developed...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail... nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

...protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes... I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein Guide Expression Vectors from...decades of trying to address these challenges, researchers have amassed a considerable molecular tool box...

...Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters...Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. C) Quantifications...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into Alzheimer...editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors...motif to stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to...38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use recombinant antibodies... the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid...

.... Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative Meidince - Understanding and...Balancing research with medical practice as an MD/PhD. Darrel does 20% clinical work 80% research and was... but later got obsessed with research. Chapter 3: How all basic research ends up being translational. ...for Regenerative Medicine accelerates research. Chapter 5: Research currently under way in the Kotton Lab...Chapter 8: The importance of a mix of research approaches. Why researchers should have a mix of high-risk, ...at Boston University Medical School. Darrell’s research focuses on the lungs, and, among other projects...Regenerative Medicine, and within the biomedical research community. Along these lines, Darrell recently...

... state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a single gene to cells. ...crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as ...we decided to write this quick guide to help researchers choose the probe for their needs. You’ll find...for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility to be used as internal tags due... normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was...biosensors, and optogenetic tools. Follow her recent research @daria_gift_shch.     References and resources...

... lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9 and further...2016 was an exciting year for genome engineering research. A variety of new tools came out including the...