We narrowed to 79 results for: CHL
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TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after...
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Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m.... Susanna Bachle... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m...., Chug, H., Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....Additional Resources Zhang lab Cpf1 blog post on Benchling ... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M. (2019). Adeno-Associated... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m.... Susanna Bachle... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc Sze, Victoria McGovern, and Julie... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....Snell KD, Seo JS, Chua N-H, Strano MS (2019) Chloroplast-selective gene delivery and expression in planta... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....hsdR17(rK- mK+) XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....27966527. PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m.... guide you in gRNA design. We have been using Benchling, but there are many other gRNA design tools available... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....kits are quick, easy, and don’t involve phenol-chloroform. The default option is to use the kit that ... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting...