...abilities we often hear that scientists should acquire during their PhD and postdoctoral training. Addgene executive...different types of people both above and below you. During my time at the NPA, there were many changes to ... hands-on- and role-playing exercises were held during the weekend. I attended multiple CLIs and benefited...

...contamination, use appropriate decontamination procedures (Korte et al., 2021) for all disposable items... iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their...release of functional vector into the culture medium during adeno-associated virus manufacturing. Human Gene...

... steps, until its connection with DNA is broken during cross-link reversal (not shown in diagram). Cross-links...in chromatin conformation, like those that occur during development and cellular differentiation. Using...the Xu Lab tracked enhancer-promoter interactions during erythroid differentiation. CAPTURE 2.0 captures...

... Protocol Pages Agarose cloning method procedure The procedure (illustrated in the YouTube video below...isolate colonies of antibiotic-resistant cells; during the time required to grease, place and fill a dozen...

...other side), you can check by using two membranes during your transfer instead of one. If you see signal...you had air trapped between the membrane and gel during the transfer, while dark splotches could indicate... mixed blocking buffer, or insufficient rocking during incubation steps.  Many errors can arise from technical... If you’re seeing high background or low signal during the imaging process, try adjusting your exposure...

...producer cell membrane, and acquired as an envelope during exocytotic budding, as the viral particle emerges...transfer of RT, which would actually occur in step 2 during uncoating. After uncoating, reverse transcription... integrated by the viral integrase protein (IN) during step 3 (King et al., 2008) (Hu & Temin, 1990). ...

... caused by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization...occurs, due to a reduction in target concentration during expansion. One group has found that using Fab fragment...

...to follow an exponential decay model, with dips during weekends and significant holidays. Figure...likelihood by the corresponding factor 𝜙z if t is during event z. The "events" we considered for our model... Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find plasmids...

...cross-react with samples, eliminating them from the procedure removes one potential source of cross-reactivity...antibodies that work well together. Moreover, the procedure is longer than alternative ELISA methods, especially...peptide. The positive control will confirm that the procedure is working and should always be included.  For...

...system to partition themselves to each daughter cell during cell division. They don’t simply rely on chance...CBP), and a partition NTPase (Schumacher, 2012).  During cell division, CBPs bind to the centromere-like...replication that occurs for some types low copy plasmids during cell division simply does not allow for enough ...

...creativity is absolutely a transferable skill developed during PhD training. Creativity is required to develop... need ahead of time and mute your notifications during. (Boyes, 2019) If you’ve taken the time to do this... were asked to create a device to help students during an interview. When they were instructed to use ...

...contains a plasmid with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into...transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...other aspects of the transient transformation procedure (Mortensen et al., 2019). Our method might provide...

...the host genome via the viral proteins packaged during production (read our blog post on the retroviral...time, IDLVs are ideal. As the episomes are lost during cell division, the expression of the delivered ...activity yet fail to integrate viral DNA efficiently during infection. Journal of Virology, 70(2), 721–728....

...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular... used. These dyes are incorporated into the DNA during DNA synthesis and then diluted with subsequent ...negative live cells can still be easily gated on during the analysis. Conclusion Congratulations! You made...

...progress), and I know that I’ve blocked out time during my day according to my priorities, I have a pretty...-world example, let’s say I’ve blocked out time during my day to write a blog post, edit other blog posts...leave your phone in your bag on “Do Not Disturb” during the times you’ve blocked out for other activities...

...then used to measure the frequency of each variant during the selection process. Each variant is assigned...then both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...direction, both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...

...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest...manufacturer to ensure that you’re cutting for the proper duration and using the proper conditions. Pro Tip! If you...migration speeds; after running the gel for some duration of time, these differently sized pieces will be...

...internationalization and there is a lack of expert mentorship during undergraduate and graduate studies. Students also...Valencia, Spain) as the most improved trajectory during the pilot, and Joaquín López (MIT, USA) as most...experience, and new professional capacity generated during this process result directly in positive impacts...

...minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous...  Some classes of neurons transiently express TH during development or have low levels of TH mRNA without...

...that are generated (while talking or pipetting) during working in the laminar airflow are also among the...aerosols generated will enter the culture medium during subculturing processes or from the air if the cell...drawbacks while considering this treatment as the duration of treatment can affect the cell growth and viability...