...seems to fall into place. When the balance tips, we need to look to for support and ideas to manage our stress...will be under control.” Think again. Maybe you’ll need to care for an elderly family member, want to train...side, if you are not happy at work, you probably need to find a new job. If you are miserable at home ... run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work...your family and friends to know you care, but they need to know that you have an important life too. They...exception is that men cannot actually give birth and breastfeed. Keeping this always at the forefront will go...who may wish to tell you so – towel corners do not need to match up exactly when you fold them. The same...

...labeling specificity needed and when is it not? What are the proper controls needed to pressure-test your...important. Donor labeling and the need for specificity Donor fluorophores need to go to the right place. Mis-targeted... How large is it? What parts of it move when you feed it substrate or add an essential cofactor? How many...like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding site...your molecular city). However, the acceptor doesn’t need to go to the same exact location as the donor. As...undergo FRET. If this nonspecific labeling far exceeds properly targeted donor/acceptor pairs, then any...Table). However, proper control experiments are needed to establish the dynamic range of your assay. These...

...organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...syriaca and Asclepias curassavica (Gerog Jander) Milkweed plants are known to many as the only Monarch butterfly... to treat heart disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is...Their nests are open so you can see them and even feed them RNA to do silencing in the wild. They also ...proteins. This lab has created an automated tank for feeding and maintenance that had the audience gasping. ...biologically. They have economic importance as harmful weeds. The parasitic fusion is such that the two plants...speech. Finches must learn early, use auditory feedback to learn, babble as baby birds, and have dialects...

...front door,’ the homepage is still important. It needs to express value, clearly communicate exactly what...users the ability to quickly access the content they need. All within seconds.  Addgene’s old homepage had...where they desired. It became clear to us that we needed to improve search and navigation, while also updating... to offer, and to help scientists find what they need - whether it's their first or 500th time on addgene.org... story in better ways, we realized that Blugene needed the same thoughtful treatment. Blugene has been...sure our friend matched our core branding. Blugene needs to be friendly, personable, fun, and promote our...your next experiment, that you can find what you need and feel happy and inspired to tackle your next ...

...trainee experience and how it feeds into a workforce.  The soft money system needs to be addressed.  We should...awards systems need to be radically overhauled.  Finally, the science publishing system needs to be changed...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of...years.  He spoke eloquently and vehemently about the need for change.  Dr. Bourne strongly believes this is...only know about academia and alternate training needs to be provided to take advantage of all the possible...Graham Walker, Professor of Biology at MIT, was the need to pay postdocs more.  What will the future of ...

... you'll need to decide when running your experiment, is which vector will best suit your needs? At Addgene...your choice. The first and most important thing you need to know is your expression system or environment... will determine the type of vector that you will need. You will also have to make sure that your plasmid...generating transiently transfected cells, you will not need a mammalian selection cassette (e.g. 1435 pSG5L ... its advantages; however, remember that you will need accessory packaging vectors to generate the virus...vectors with various markers and promoters. If you need to visualize YGOI on a western blot or under a microscope...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids...

...Glick from SnapGene. SnapGene was created to meet a need. While there were software tools available to biomedical...center, and make specialized controls available when needed. SnapGene has been engineered to be easy and enjoyable... iterative refinements in response to customer feedback. An example of this approach is SnapGene’s algorithm...that may be used to make fusion genes, detection needs to occur even if one or two codons are missing at...miss a common feature if the sequence differences exceed the threshold. This issue is addressed by adding...useful because it helps us find which common features need to be added to the database – we will work to fill...mismatch tolerant detection algorithm and thereby speed up the annotation process. Many of the best ideas...

...coronavirus-related deposits. Our valiant lab and shipping staff agreed to come in and start getting plasmid deposits into...industry requestors as well. This is a time when we need as much open science sharing as we can get. ...occasionally for capital equipment or resources needed to launch a new service or product. Our daily operations...entirely self-supporting. We hire more staff as we need them to meet demand and to serve the scientific ...the crisis and maintain our workforce for when we need to be working at full capacity to serve the scientific... with demand and keep new projects on track to succeed at the same time. I began approaching science funding...the community. While many were sympathetic to this need, we're incredibly grateful that the Chan Zuckerberg...

... pitching for feedback.  Your venture should continuously evolve and respond to feedback throughout its...idea, you will rarely succeed in launching a company without partners.  You need a dedicated team that...capital to get off the ground, as you will likely need a functional prototype to gain any real traction...beginning the process of launching a company, you need to determine whether or not your idea is viable....was originally planned, due in large part to the feedback we received from our network.  We started with....  After each pitch we made, we considered the feedback and adjusted our business model accordingly until...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...systems contain all the parts needed to produce proteins without the need to handle a living, replicating...essentially giving the cell all the instructions it needs to produce that green fluorescent protein through...having live cells in a classroom presents equipment needs, such as shakers and incubators, and biosafety issues...shelf-stable until water is added and protein production proceeds. FD-CF pellets are easy for students to use in...in the classroom because the students simply need to add water to express a variety of proteins. FD-CF...

...would allow you to train and acquire the data you need on the new instrumentation. Bonus reasons Visiting... institute or lab So, you’ve identified a gap or need in your research and decided you want to pursue ...pursue a visiting position. What now? First, you’ll need to find someone who is willing to host you! If there...collaborates with that can fulfill your research needs, that is probably the easiest path. Some institutes...are often great choices if they fit your research needs. What are your options if neither of these situations...familiarize yourself. Identify reagents you will need, any required training, and a priority list of experiments... reasonable, your reagents are onsite before you need them, and you are set up for success. If you are...

... outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator...are needed for the role, as this will give you an idea of what additional training you might need to obtain...also need to think of other aspects of yourself that make you stand out. For every role you need to show...Illustrator Plant Breeder Zoological Worker Conservationist Documentary Director Parasitology Botanist Nature... team? Will you be coordinating projects? Do you need computer programming skills? Other things to consider...

...read comes to this: we need others to review our experimental designs and we need to be transparent about...expect that every study should be reproducible. Indeed, researchers are pushing the limits of knowledge...estimates reaching in the billions of dollars (Freedman et al 2015). Finally, as Pamela Hines of AAAS ...best apply these technologies to your own research needs. Other companies like NEB and ATCC can provide you...). Once you’ve completed your experiment, you’ll need to analyze your results. As biological researchers...to do modified analyses on published data if you need to. Similarly, FigShare also makes it easier to ...through validation are easier to understand. The need for great stories has, however, made it difficult...

... do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or a maxiprep...either a forward or reverse orientation so you’ll need some way to verify that the insert ended up in the...overhangs or no overhangs after digestion, you will need to use a phosphatase to prevent re-circularization... just throw the digestion mixtures together. You need to isolate your insert and backbone from the enzymes...overhangs cut off of it via their different migration speeds; after running the gel for some duration of time...it looks like your ligation has worked, you will need to pick individual bacterial colonies and check ...(the more background, the more colonies you will need to pick) and grow overnight cultures for DNA purification...

...Working long distance, you need to be very self-motivated, because you need to work independently most... I applied for this summer position as I felt I needed more practical lab experience to later gain a full...if it is wasn’t a good fit for me, then I didn’t need to explain why I changed jobs. I highly recommend...I enquired about roles in this field, most jobs needed at least 3 months experience working in a developing... with a different climate and culture, and also needed to be resourceful and adapt. By the end of my three...really get to do a lot of lab work but instead really need to be comfortable with programming. Working for ...different aspects of the company and gave me the freedom to design new projects and grow in the role. I ...

...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...find your desired plasmid. In many cases, you will need a screening strategy as well. In a screen, you aren...selection. Instead, all cells survive and you will need to sort through them to find the desired clone. ...easily identify successful clones so you have to weed through fewer colonies after your experiments. As... DNA using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using... expect it to be. To get started, you will first need a primer that complements your plasmid sequence....primers can't provide all of the information you need, so you'll want to design a custom primer. Because...

...while the technical skills needed to perform GCE are essentially those needed to make site-directed mutagenesis...requirements of use. First, and most obviously, you need a non-canonical amino acid, which is usually added...the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases specific to the ncAA ...cells via plasmid. Third, a second plasmid will be needed to introduce the gene of interest containing the...including media, cell type, and growth temperature) may need to be individually optimized for each of the three...leading to system-wide effects. And on top of that you need to worry about getting high enough expression of...protocol, to optimize and share, they’re soliciting feedback from scientists on which protocols would be broadly...

...  If you’re working with a tissue sample, you’ll need to homogenize your sample before moving on to the...homogenizer, but bone or especially fibrous tissues may need extra steps.  Fragmenting  The next step is break... chromatin.  Whichever method you choose, you’ll need to reserve a small aliquot, split into two parts...antibody-bound samples. With a bead method, you’ll need to ensure you have a bead-only control alongside...Capturing), if you ask me.  Each of the three steps need to be optimized for each specific sample type. And...optimization of another. For instance, a sample may need more or less cross-linking to firmly attach the ...output assay. A longer sonication time could mean needing to increase crosslinking to counteract the effects...

... only a subset of base substitutions.  The need for speed: base editing before Cas lets go Figure...domain lets go of the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to...of gene III to base editor activity and editing speed and consists of the following components: A plasmid...variant, the phage is not infectious. The editing speed dictates which phage variants can propagate faster...of gene III to base editor activity and editing speed. Created with BioRender.com. Introducing ABE8e...higher than ABE7.10. However, the increased editing speed and targeting scope of ABE8e comes with a price:...naturally occurring enzymes and functional domains speed up the development towards faster and more precise...

...functioning lab, but it is for a scaleable lab. When you need to track the location, quality, growth, and legal... In order to get a plasmid to your lab bench, we need to (this is a little simplified): Find the appropriate...require starting over entirely, and our software needs to handle these cases. The following code examples...we’re sure our techs could figure it out if they needed to, they’d have to individually keep track of any...whole procedure. Other steps fail “soft” they simply need to be repeated. We also now have a good interface...easier, the time and capital cost of getting up to speed with a setup is almost always worth it. Because ...and tube model, we can interchange them easily as needed. In this case, the physical considerations of the...