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...information from requestors and depositors, and identifying trends in our own repository data, giving us significant...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks ...order high quality viral vectors from the Addgene repository can save researchers significant time and removes...Cumulative AAV preps available in the Addgene repository each year from 2016–2023. B) AAV preps distributed...serotypes. In fact, the 879 viral vectors in our repository are produced from only 413 plasmids containing...different types of tools scientists were using/depositing. Our viral vectors data is less amenable to such...GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene...

...potential new deposits, and to meet with scientists throughout the world to encourage them to deposit. However...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...reach out to will end up depositing, we hope this campaign will expand the repository in ways that will be...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...

...use of both when making plasmids. Use repositories Repositories such as Addgene help share materials quickly...After the great Plasmid Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid...in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations for any...clear that community engagement with and use of repositories like Addgene provides large-scale savings. How...and the 2018 numbers for consumable costs, our depositors have helped us save the scientific community ...on Addgene.org Join the community of Addgene depositors here Check out our special collection pages to...find plasmids Search for a plasmid in Addgene’s repository ...

...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations...GFP. They then tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells ...tested in retinal electroporation studies, DsRed reporter expression was induced in ~76% of GFP+ cells, ...

...fluorescent reporter, directly couple silencing of your gene target to silencing of the reporter. This is...fusing your gene target sequence to a fluorescent reporter sequence (discussed below). Examples of these ...contains a target sequence against the fluorescent reporter in an inverted repeat orientation. Therefore, ...sequence against your gene(s) of interest and the reporter. This approach greatly enhances experimental throughput... observable phenotypes quantified. Fluorescent reporter-based RNAi constructs have been designed and widely...processes to isolate RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological parameters...identified as undergoing RNAi through the fluorescent reporter and manually extracted. This process is tedious...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...in a protein.    Cells expressing an RFP reporter plasmid for recombinase activity were exposed ...introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered...library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library here A new...  Find the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible CRISPRa system...

...unique skills and strengths (you will likely have reports with different levels of experience), find out ...tasks before I was a manager! Second, my direct report was not engaged because I only left him/her with...you’re delegating a recurring task to a seasoned report, there should be questions! Remember that delegating...itself is counter-productive. I always assume that reports want to succeed at work and providing feedback ...feedback is needed for that. In many cases reports are not aware of issues until you bring them up. I wouldn’...wouldn’t go as far as saying that your reports will be grateful for the feedback, but at least you can start...someone’s hard work and reward a job well done. Your report will (needs to) feel valued. Talk to your manager...

...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ... 2A element (depending on the placement of the reporter gene). Addgene's collection offers a variety of...multicistronic element(s), or you could replace one of the reporter genes above with your gene or genes of interest...equivalent levels upstream of an IRES fluorescent reporter for easy detection.5  Just released: Addgene's...

... our viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21...vectors that can constitutively express JEDI-2P, deposited by the St-Pierre lab. Also of interest is the ...check out Addgene's Data Hub to view viral vector reports from other users. And don't forgot to use the link...

... people usually report? Addgene and other viral vector manufacturing facilities report the physical titer...cell transduction assays. Wild-type AAV2 has been reported to have a near-perfect physical-to-infectious ... However, for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:...(“full”) (Zeltner et al., 2010), while another reported that only 20% of recombinant AAV2 particles were...vector preparation protocol, and we are pleased to report that over 95% of viral capsids in representative...AAV aliquots. Our titer values (circled in red) report physical titer, which is measured by qPCR. ...nonsense to publication quality data." Scientific Reports 7 (2017). PubMed PMID: 28546538. PubMed Central...

...molecular brightness (Table 1).  NIR reporters and biosensors NIR reporters can tell us about cellular events...higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., 2016), since...fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found to be a...E., Sivanesan, D. & Michnick, S.W. An infrared reporter to detect spatiotemporal dynamics of protein-protein...Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo imaging of protein-protein interactions...evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu, D. et al...

...research. If you’d ever like to deposit a kit, please contact us at depost@addgene.org. ...of the many useful kits you can order from the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System...library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid set 3000 Series Confronted...strengths centered within GFP/RFP bicistronic reporters characterized in Cambray et al., 2013. These terminators... an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37. pCri...quantitatively determine allele frequencies across timepoints or populations. This kit consists of a 48 plasmid...fusions. Some applications of PUF protein fusions reported in the literature include RNA detection, RNA cleavage...

...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ...commercially available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide ... Calculator app for iPhone Reagent and Data Repositories These links can help you find experimental and...for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX...IBEX Knowledge Base: A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies...human, and marmoset brain IHC.  FPbase: A data repository for fluorescent proteins and their properties...

... The code and the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare... or biological material, can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info...published work meets the accepted standards of reporting in the discipline and reduces the dissemination...Arabidopsis.info). These repositories will ensure that the results of your colleague’s hard work will be maintained...article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular biology...

... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from...is currently using VAMP-seq to study vitamin K epoxide reductase (VKOR) variants. VKOR is an enzyme critical..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...artificial leaf, Bernach will use the CUSPER bioreporter such that fluorescent proteins expression is ...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...using TIMER and an unstable fluorescent protein reporter under the control of promoters that control cell...

...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.        A retroviral mouse...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ...cells. These changes were larger than previously reported for other organisms, and the Gottschalk group ...

...this list, that does not necessarily mean the repository does not have it. That said, if you know of a... don’t hesitate to start the process! Addgene depositors who study chromatin The PIs linked below are ...investigators in the chromatin field who have deposited with Addgene, but you’ll find many great tools...Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin Cheryl Arrowsmith James...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors or not, your time is valuable ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find ... a cleavage free manner. The dCas9-SSAP system reports an efficiency of up to 20%, comparable to commonly... For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%, both ...using mStrawberry, localization peptides, and reporters that drive the expression of the fluorescent protein...response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman...

...is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute...for Addgene to present our own data on plasmid deposits and distirbution for these fast moving fields....Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...presented a poster with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, ...next hot molecular biology technology? Have you deposited your plasmids yet?      ...

...in genome target sequence into EGFP reporter. Target DNA reporter system: Can evaluate component delivery...mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these outcomes can... that would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum...promoter is typically used for gRNA. May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive...published as CRISPR testing tools: Traffic Light Reporter System: Can evaluate lentiviral component delivery...