We narrowed to 77 results for: EXO

1. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ...Dustin, J., Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor...

2. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...endogenously available sister chromatid, though exogenous DNA can be used as well. Below are some considerations...

3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ...requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA into the target...

4. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz Codon Optimization  ATUM DNA2.0  IDT...

5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...or alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search...

6. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...and found the protein La (a small RNA-binding exonuclease protection factor) promotes editing (Yan, J et...

7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...sequences to select from - in many cases several exons’ worth of DNA. Any ∼20 nucleotides of DNA can serve...

8. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   Published

   Oct. 5, 2021, 1:15 p.m.

   ...transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence of ...

9. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

   Type

   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...likely a rate limiting step, but optimization of the exon-splitting site sequence can enhance the rate of ...

10. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...earlier system.  Rather than overexpressing an exogenous methyltransferase for epigenetic silencing, which...

11. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...hybrid between a genetically encoded tag and exogenously added components. For a review see Lotze et al...

12. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...new information into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency...

13. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...BRET reporters produced bright signals without exogenous excitation and with OgNLuc being the brightest...

14. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...regions of open chromatin, especially at promoter or exonic regions. If the cell’s chromatin structure undergoes...

15. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ... causes a large deletion that removes the entire exon that codes for the epitope recognized by your antibody...

16. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...be used on tissue from whole animal models, no exogenous component required (e.g. dsODN), doesn't require...

17. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...protein is another way to avoid exposing cells to exogenous genetic material. Proteins are delivered to cells...