We narrowed to 237 results for: FAS
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TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....industrial labs accelerate research and to help find a faster solution to the COVID-19 pandemic. Though we’ve...instrumental in achieving our mission to help science move faster in the middle of a global pandemic. And the reach...
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Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....throughput and faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...each other practice meeting people – compete for fastest to set up a coffee date or most cards collected... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative...30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....enzyme. Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell...cyanobacterium Synechocystis sp. PCC6803, and the fast growing strain Synchococcus elongatus UTEX2973, ... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....pandemic quickly and openly. By sharing results faster and more broadly, scientists can receive recognition...Preprints are all about sharing scientific knowledge faster and more openly — which is also our mission here... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....to mankind. And you probably understand how much faster it would be for an expert in the field to read ...students were fired up and enthusiastic and needed a faster presentation with more information than normal.... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... lines such as CAR-T cells. Plus, this method is fast! These editing frequencies can be achieved with ... to the original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... individually assembles the reads into a single FASTA sequence that our QC scientists can easily analyze...each sample. About 24 hours later we unload the FASTQ data from our instrument and begin the analysis.... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....CRISPR plasmids New items from the viral service A fast blue-light inducible Cre for precise control of ... can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as 2 hours. Sheets, MB... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m....responses asking for more specifics. We can help you faster if we get as much information as possible about...one. Though an initial response should be fairly fast, getting the full answer to a complicated question... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....cjBlue, meffBlue, aeBlue, amilCP, tsPurple, and gfasPurple) (Figure 2). Figure 2: Chromoprotein expression...combines the most desirable features: intense color, fast maturation, and low fitness cost yet. However, many... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts of... with a single size insert, liquid media can be faster and easier to work with. In either case, it can... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the overnight...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM)... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....syringe filter method is not only cheaper, but also faster and more flexible than commercial kits. DNA gel...bead-based kit, this cellulose-based method was faster, cheaper, and had a similar level of sensitivity... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....bacterial and yeast cells are cost-effective and faster-growing than mammalian cells, but mammalian cells...successful. Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....first generated a multi-fasta file of the hg19 genome using Bedtools getFasta. These regions and their... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage...means something fun is going on and to get there fast! Spread word of your plans by email, gong, evite... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....making experimental workflows considerably easier, faster, and more efficient than previous methods. Still...Sanger sequencing-based software tools and is also faster and easier to use. ICE is completely free for everyone... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m.... we contracted with so we can always offer the fastest shipping. However, there were issues that persisted...able to better understand the barriers to easier, faster, more reproducible science and learn what Addgene...