...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...

...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

...original CAPTURE method The original CAPTURE method requires the creation of a stable cell line that co-expresses...expression. Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ...

...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...relatively even distribution of target gene expression (EGFP, in the depositing manuscript) across the brain....