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We narrowed to 172 results for: KIT

Showing: 61 - 80 of 172 results

  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove...

  2. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and every contributor... for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a ...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent Protein Kits Browse the Fluorescent Protein... recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging...offers for tagging a single genetic locus, the toolkit also offers repair templates for tagging proteins... elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model...obtained as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging... S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP... Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...used in this study.  The zCas9i cloning kit is a plant cloning kit that contains a series of vectors and...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found across all TCGA...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in ...

  6. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...

  7. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...mycoplasma contamination using mycoplasma detection kits. Cell line is maintained for ~20 passages before...prepared using endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode ...

  8. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...Lentiviral Expression Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...working, consider using a subcellular fractionation kit or a lysing protocol developed for isolating proteins...based lysing protocol Subcellular fractional kit or lysing protocol Not enough target Run protein...Chemilumenescence: use a more sensitive exposure kit Too much signal Antibody dilutions too high...Chemilumenescence: use a less sensitive exposure kit No signal variation in highly expressed proteins...

  11. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...targeted markers. Some commercially available fix/perm kits allow for labeling of cytosolic proteins, as well...located inside the nucleus, a different fix/perm kit is required (to be precise, the fixing solution is...

  12. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate ...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...in this blog post. The pDGE Dicot Genome Editing Kit from the Stuttmann lab contains a variety of Agrobacterium-compatible...from the Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the...described above, Addgene carries several useful CRISPR toolkits for creating plant expression plasmids, including...

  14. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ... the paper in bioRxiv Find the GeneWeld vector kit CasX, a small Cas protein, is active in E. coli ...
Showing: 61 - 80 of 172 results