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We narrowed to 169 results for: Library

Showing: 61 - 80 of 169 results

  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...screening markers. Capability to construct a strain library with different gene dosages to facilitate swift...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https:...example, can be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools...structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding...

  5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...files of research articles. It’s your own digital library for the full-text version of useful papers. For...options for helping you manage your own digital library. Check out Papers and Mendeley – these programs...options for searching and sorting your own digital library as well as some integration with internet-wide ...

  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids... Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources: CRISPR Plasmids...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    .... Diversity is introduced into the cap plasmid library by inserting short DNA sequences that contain all...amplification using pre-designed primers. By using this library to infect an animal expressing Cre in a cell type... to reach the brain. Delivering the CREATE AAV library via IV screens for capsids that are able to cross...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...created mTFP1 by transforming E. coli with a gene library of approximately 484 variants, including some potential... potential tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis...

  11. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution tracking...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes...to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments...experiments. Get the full details on how to make these libraries from the post and the associated publication. ...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate ...

  17. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... has been working on developing CRISPR pooled libraries for use in genetic screens and has contributed...Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of the Addgene Advisory...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses... O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11...
Showing: 61 - 80 of 169 results