...species, even from completely different kingdoms of life. That’s convenient for researchers who want to do...episomal vectors is to include viral oris to drive amplification, which helps increase cellular uptake, but this... DNA intercalator, which affects all kingdoms of life. Of course, that includes you. Besides the risk ... you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make your experiment...

...leading to genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...attributions (from top to bottom) Database Center for Life Science (DBCLS),  National Cancer Institute, Public... Skloot, Rebecca, and Bahni Turpin. The immortal life of Henrietta Lacks. New York: Crown Publishers, ...

...fusion proteins Biotin is an essential vitamin for life Although biotin is most commonly known for its role...essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants, most bacteria...sites found on avidin are also ideal for signal amplification during these experiments. During detection, ...bound to your protein of interest leading to amplification of the reporter signal and easier detection....

...by scFv antibodies fused to sfGFP. This system amplifies the intensity of fluorescence signal and enables...gene of interest. The gene to be expressed is amplified using a gene-specific forward primer containing... based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a ...ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221 can be expressed either...(MuLE) system from the laboratory of Ian Frew simplifies the process of creating complex polycistronic...iterative, chaining-based cloning method that simplifies the process of constructing specialized fluorescent...destabilizing sequences were added to shorten the half-life of both the luciferase mRNA (3’UTR AUUUA [5 consecutive...

...language. During the recession there were many qualified scientists with experience and a second language...carry out my dissertation research in a lab in California. I felt then, and still feel now, that it is ... do. I had taken advanced virology classes in California and enjoyed them, so I applied for a role at ... day planned out. This allowed for a better work-life balance compared to working in research. My previous...

...biomedical scientists and had no idea what the daily life of a scientist was like. The word “scientist” conjured...those scholarships that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots... feel less confident in their abilities and qualifications. Studies on imposter syndrome have also found...and ended up with the award. We are often more qualified and capable than we know or believe we are. Funded...

...the scoop on the ups and down of graduate student life. Senior graduate students can sometimes be elusive...and talk to us and wish them the best of luck in life beyond graduate school. Ben J. Vincent is a Ph.D...fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome biology and...

...that deserve a second look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger...growth medium, these apps can help make your lab life a bit easier. Since the post doesn’t provide an ... lab life...

...was certain I would not end up in a life sciences career but life has a way of making unexpected decisions...what it was like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke...

...reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer...

...past few years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible...information on black screens, but not to know even the life cycle of the creature they deal with.” So photography...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert...

...bold. You don’t have to have all of the listed qualifications to apply for a position.  A job description...application). Beware the myths of scientist “overqualification” but it may not be productive to apply for...resumes The cover letter must address how you are qualified and why you are interested specifically in the...

...articles openly available on PubMed Central (also eLife, PLOS and Figshare). Some use cases include: Cataloging... collections from a large section of open access life sciences data. You can use ReFigure to coherently...we can only connect figures from PubMed Central, eLife, PLOS and Figshare but we are working on expanding...

...that I belonged there. Addgenies had a good work-life balance and were content. As a worker, there are...them. Addgene gave me the chance to become a real life scientist. Addgene gave me the chance to grow into... arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it any other...

..., allowing me to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify...once said: “There are only two ways to live your life. One is as though nothing is a miracle. The other...quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters....

...with and/or requests materials from Addgene can qualify. We also reward improvement against one’s own record...Hospital  Brandeis University  Broad Institute  California Institute of Technology Cold Spring Harbor Laboratory...University of British Columbia  University of California, Irvine University of Massachusetts, Worcester...

...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

...researcher on parasitoid wasps; and an essay on the life of Srinivasa Ramanujan, to be precise) and remarking...neurological health risk. Having played the sport my entire life, I was curious as to what the research had to say...chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry. A research...

...multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...

...which limits its use for imaging a protein’s entire life-cycle. This makes it nearly impossible for scientists...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...