...paper in Nature Methods offers the first true two-color pair. We'll be sending it to Addgene in a matter...Can you tell us a little more about this new two-color tool and what it can enable? Boyden: There are many...perhaps compete against each other. Getting two-color stimulation has been a holy grail of the field. ...

... where 1000 years ago the hero lost and the Dark Lord rules all, the books center around a cast of endearing...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

...single mRNA over time. Both are helping researchers explore the complexity of translational control in cellular... and translated RNAs appear red. To enable this color switching, TRICK requires the following components...translocation rate of ~3.5 codons/second. They also explored the effects of 5’ UTR variants in translation ...

...services! Many thanks to Joe Mellor, our guest writer from seqWell Inc. Joe Mellor is the Founder and CEO of...This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR...

...Here, we look at the origins of the system and explore advances that have happened since our original ...of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia Research Campus to ...affinity for glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR...

...special equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...to a particular strain of norovirus, it can be tailored for the detection of other norovirus strains and...

...BioBuilder embraces new opportunities for scientific exploration, discovery, and collaboration. One module currently...and the new BioBuilder module allows students to explore how engineering fields rely on rapid prototyping...the lab as a starting point into further STEM exploration.  Further offerings for both STEM teachers and...

...Rosella fluorescent properties Rosella is a dual color-emission biosensor named after the brightly-coloured... or lysosome. See Table 2 for a summary of what color fluorescence Rosella emits based on its localization...fluorescence emission. Localization pH Emission Color Cytosol ~7.5 Red and Green Mitochondria ~...

...antibodies that produce detectable signals like light or color. In this post, we’ll go over some of the common ...blots and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...filtration.    There is, of course, a lot more to explore in the world of antibody conjugation. But hopefully...

...all the plasmids from the associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2...ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular fluorescence complementation...(split HaloTag) provides maximum flexibility in color labeling with exogenous fluorophores, which can ...

...over the past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR)...solutions. The 2015 Boston FoR meeting was designed to explore areas where further data is necessary to determine... 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost generation ...

...some cases I couldn’t tell if the bags contained flora or fauna. I tried to find out what certain ingredients...and more are being reported. Zhou Songyang from Baylor College of Medicine and Sun Yat-sen University ...cas9 inducible knock-out system. His lab is also exploring the use of cas9 proteins from different species...

...the end tip of their abdomens, males have black coloration whereas females do not. Also, males have sex ...first emerge, their bodies will be very light in coloration and can look very plump. On the side of their...research, you’ll find more resources that may be more tailored to your field of study. 5 great resources to help...

...inside a cell with the images from the Allen Cell Explorer (1). When studying the function of a protein or...the human eye and are indifferent to subjective color intensity perception. Open source image analysis...FP you should use and how to select FPs for muli-color imaging. Furthermore, one must bear in mind that...

...use a reagent like Qiagen’s LyseBlue, which uses color to show you if lysis is incomplete. Protein expression...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ...often require a longer growth time. Note: Julian Taylor-Parker contributed to the writing of this post....

...on gene transfer, from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. ...in plants because of the auto-fluorescence of chlorophyll, so we looked at newer fluorescent proteins that...gotten some tools back from Addgene recently to explore their use in plants, including FRET tools, double...

...involved. For example, an animal moving around to explore the environment has a different motivation (and... illuminated ones (risky). They also prefer to explore novel environments by initially moving along a ...risky bright place, driven by their motivation to explore novel environments, rather than being inhibited...

... be more precise and more repeatable than qPCR (Taylor et al., 2017). However, because purified AAV preps...components, ddPCR may not be an improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean...: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain...

...month, giving you a plethora of opportunities to explore! Again, attending one of these fairs is a great... know who is going to be there, so that you can tailor your CV accordingly. Make it stand out with key...graduate schools will even pay for you to attend!), exploring a job fair, and utilizing your local career development...

...exchange vector. This vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of ...products. To allow manipulation of naturally chloramphenicol-resistant strains, we have also included versions...exchange system, he is so excited to use it to explore how hospital-associated Gram-negative bacteria ...