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We narrowed to 316 results for: Lor;

Showing: 61 - 80 of 316 results

  1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...B978-0-12-800947-5.00004-1 Taylor, M. W. (2014). What Is a Virus? In M. W. Taylor (Ed.), Viruses and Man:...are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t even decide...

  2. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    .... They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell behavior...GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations responsible...disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers found...

  3. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...paper in Nature Methods offers the first true two-color pair. We'll be sending it to Addgene in a matter...Can you tell us a little more about this new two-color tool and what it can enable? Boyden: There are many...perhaps compete against each other. Getting two-color stimulation has been a holy grail of the field. ...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ... where 1000 years ago the hero lost and the Dark Lord rules all, the books center around a cast of endearing...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...single mRNA over time. Both are helping researchers explore the complexity of translational control in cellular... and translated RNAs appear red. To enable this color switching, TRICK requires the following components...translocation rate of ~3.5 codons/second. They also explored the effects of 5’ UTR variants in translation ...

  6. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...services! Many thanks to Joe Mellor, our guest writer from seqWell Inc. Joe Mellor is the Founder and CEO of...This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...Here, we look at the origins of the system and explore advances that have happened since our original ...of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab at HHMI Janelia Research Campus to ...affinity for glutamate), that came in three different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR...

  8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...special equipment. What’s more, detection is based on color changes to the assay paper, so no special equipment...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...to a particular strain of norovirus, it can be tailored for the detection of other norovirus strains and...

  9. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...BioBuilder embraces new opportunities for scientific exploration, discovery, and collaboration. One module currently...and the new BioBuilder module allows students to explore how engineering fields rely on rapid prototyping...the lab as a starting point into further STEM exploration.  Further offerings for both STEM teachers and...

  10. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Rosella fluorescent properties Rosella is a dual color-emission biosensor named after the brightly-coloured... or lysosome. See Table 2 for a summary of what color fluorescence Rosella emits based on its localization...fluorescence emission. Localization pH Emission Color Cytosol ~7.5 Red and Green Mitochondria ~...

  11. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...antibodies that produce detectable signals like light or color. In this post, we’ll go over some of the common ...blots and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based...filtration.    There is, of course, a lot more to explore in the world of antibody conjugation. But hopefully...

  12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...all the plasmids from the associated paper:   Color Base FP Description Article PI Blue EBFP2 EBFP2...ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular fluorescence complementation...(split HaloTag) provides maximum flexibility in color labeling with exogenous fluorophores, which can ...

  13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...over the past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR)...solutions. The 2015 Boston FoR meeting was designed to explore areas where further data is necessary to determine... 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost generation ...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...some cases I couldn’t tell if the bags contained flora or fauna. I tried to find out what certain ingredients...and more are being reported. Zhou Songyang from Baylor College of Medicine and Sun Yat-sen University ...cas9 inducible knock-out system. His lab is also exploring the use of cas9 proteins from different species...

  15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...the end tip of their abdomens, males have black coloration whereas females do not. Also, males have sex ...first emerge, their bodies will be very light in coloration and can look very plump. On the side of their...research, you’ll find more resources that may be more tailored to your field of study. 5 great resources to help...

  16. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...inside a cell with the images from the Allen Cell Explorer (1). When studying the function of a protein or...the human eye and are indifferent to subjective color intensity perception. Open source image analysis...FP you should use and how to select FPs for muli-color imaging. Furthermore, one must bear in mind that...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...use a reagent like Qiagen’s LyseBlue, which uses color to show you if lysis is incomplete. Protein expression...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ...often require a longer growth time. Note: Julian Taylor-Parker contributed to the writing of this post....

  18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...on gene transfer, from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. ...in plants because of the auto-fluorescence of chlorophyll, so we looked at newer fluorescent proteins that...gotten some tools back from Addgene recently to explore their use in plants, including FRET tools, double...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...involved. For example, an animal moving around to explore the environment has a different motivation (and... illuminated ones (risky). They also prefer to explore novel environments by initially moving along a ...risky bright place, driven by their motivation to explore novel environments, rather than being inhibited...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ... be more precise and more repeatable than qPCR (Taylor et al., 2017). However, because purified AAV preps...components, ddPCR may not be an improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean...: 20486768. PubMed Central PMCID: PMC2957240. 5.Taylor, Sean C., Genevieve Laperriere, and Hugo Germain...
Showing: 61 - 80 of 316 results