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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 180 results for: PID

Showing: 61 - 80 of 180 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...other biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized...1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...amine reactive dyes. DNA binding dyes, such as propidium iodide, can only enter cells with disrupted membranes...

  5. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments... is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that these...

  11. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...are shared. The scientific landscape is evolving rapidly, and with it, the needs of researchers worldwide...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant, Drosophila...

  16. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...genome-wide off-target DNA editing. Using several rapid and cost-effective screening methods, David Liu’...
Showing: 61 - 80 of 180 results