We narrowed to 181 results for: PID
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TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....antibodies (Ahmed et al., 2012, Bates and Power., 2019): rapid blood clearance, which is useful for imaging applications...their small size (Bates and Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic...
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Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently become...Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural... -
Which Fluorescence Microscopy Techniques is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....just those in the focal volume. This leads to more rapid photobleaching and a reduction in signal intensity... 2-photon microscopy, lightsheet microscopy is rapidly becoming the preferred technique for many of these... -
Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....single step by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases...amine reactive dyes. DNA binding dyes, such as propidium iodide, can only enter cells with disrupted membranes... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...we now know that T cells are able to crawl more rapidly than any other cell type in the body! Intravital... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments... is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled and that these... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m.... before it, offers an interesting view into how rapid innovation affects the research community. I’ve ...believe the future of CRISPR software is promising. Rapid innovation in this space has put us in a good position... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....are reflecting on how to help scientists in this rapidly changing environment. In particular, we are working... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m..... TALENs - Genome engineering methods improved rapidly in 2011, from zinc finger nucleases to transcription... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....are shared. The scientific landscape is evolving rapidly, and with it, the needs of researchers worldwide... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....score as hits in pooled screens, facilitating more rapid follow-up. We hope the biological community will... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....identify those that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant, Drosophila... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m.... Grants that support us in our mission. While rapidly expanding our COVID-19 resources has been our focus... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....genome-wide off-target DNA editing. Using several rapid and cost-effective screening methods, David Liu’... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....S A. 2014 Mar 17. PubMed) Peptide tag forming a rapid covalent bond to a protein, through engineering ... -
Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....various tools, this may happen over email or using rapid communication tools such as Slack. Share data and... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m.[email protected] to submit their published data for rapid curation review and upload. In addition to our main...