...practices, their opinions towards ELNs, and their desired functionalities i.e. main priorities of different...it as very important. One comment reflected the desire for a flexible generic solution, rather than an...

...generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure...PCR that uses mutagenic primers to introduce all desired codon changes (step 1 in the image above). A second...

... While it is certainly possible to select for desirable traits using traditional plant breeding approaches...modification of known or suspected genes that regulate a desired phenotype. In fact, CRISPR has already been used...

... of target site is far more constrained by the desired location of edit; efficiency decreases dramatically...to optimize numerous parameters to generate the desired edit. The wrench: Gene activation and inhibition...

...backbone) contains the register. Depending on the desired application, the inducible promoters we used to... be replaced by sensors that correspond to the desired inputs. Such sensors do not necessarily have to...

...for cells that had successfully incorporated the desired mutation, and then derive mice from these engineered...CRISPR is much more efficient at generating the desired mutant offspring, with 85% success compared to ...

...codon (often amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural...so small, one can insert them in virtually any desired location in a target protein, though predicted ...

...Unpublished or pre-publication plasmids" and set a desired distribution status. You can submit "Published ...general description of the deposit. Select your desired distribution status: Hold for publication: we...

... a gene-specific forward primer containing the desired restriction site (the MCS has several options) ...specified order using Multisite Gateway cloning. A desired insert is cloned using traditional restriction ...vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate reporter...

...IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system...

...targeting specific sequences of RNA is highly desirable for extending the complexity of genetic circuits...

... also made it hard for users to get where they desired. It became clear to us that we needed to improve...

...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...

... if there are no available guides close to the desired mutation site. Quick tips for nickase design Ready...

...cells or when a high degree of signal to noise is desired. It also provides an excellent additional measure...

...Bonus: It displays similar on-target activity to siRNA, but with far fewer off-target effects! This characteristic...

...especially if downstream antibody detection is desired… and yes, we have an antibody for that!   ...

...research, SciFund is really motivated more by the desire to help close the widening gap between science ...

...with the majority of cells failing to develop the desired identity. The development of single cell technologies...

...designing a repair template that contains your desired edit, and I want to make sure that I (and Addgene...