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We narrowed to 351 results for: SUA

Showing: 61 - 80 of 351 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric population...However, here it appears that the culprit for these unusual cases was either 1) a physiological effect of SARS-CoV...transgene) instead.    Although the late genes are usually present in the transfer plasmid for the majority...

  2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...interests include proteomics, machine learning, and visualization for big biology. GPS for the genome We can think...something like Google Maps to help us identify and visualize addresses. That’s where enhancers and genome math...result is a heterogenous population of stem cells, usually housed in a single flask. Through a bit of stochastic...

  3. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...steal all their food from other plants and are unusual enough to be interesting biologically. They have...squid has an extreme number of nerve cells and an unusually high level of RNA recoding in the brain. RNA editing...each other in captivity. Sticklebacks demonstrate unusual parallel adaptation to disparate environments so...

  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There...Unfortunately, the symptoms of mesothelioma are usually very similar to the symptoms of other diseases,... meaning that by the time it has caught it has usually started to spread (metastasize) and turned into...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...and temporal resolution, making it ideal for visualizing gene expression in vivo.  But what ultrasound...genes that, when expressed, enable cells to be visualized under ultrasound imaging. Expression of ARGs ...first-generation mammalian ARGs (mARGs) enabled ultrasound visualization of in situ mammalian gene expression in deep...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...divided into two halves that are distinguished visually through different patterns and the animal only...the opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software...electric foot shock that has become associated with a visual or auditory cue. The hippocampus has long been ...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... luck! You don’t need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion...deletion large enough that it can be visualized by running your PCR on a gel. It’s still paramount that...amplicon isn’t too large so that you can easily visualize the size shift you anticipate. A good rule of ...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...trained to have these types of negotiations. They usually turn into “win-lose” negotiations. This leads to... together. The problem is that the discussions usually focus on the numbers, rather than the necessary...weight. Therefore, too much focus on money can dissuade a productive negotiation. A more productive discussion...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following...however, is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent.... This means that, for gene editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...ways to ultimately achieve success, we find it is usually faster and easier to do the validation first.  ... efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes...titer” is required for efficient AAV transduction, usually without any numbers for context. That’s because...

  11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... history, fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening...these fluorophores to enable highly specific visualization of cellular components, typically a protein ...Alexa Fluor 488, and thus it is more difficult to visualize the stain from the background. Dyes that can pass...

  12. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...that many people honor. Talking with strangers is usually challenging but does get easier with practice. ... by meeting with people working in diverse and unusual fields. My favorite final trick is to end each ...

  13. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs in vitro, you can send us... have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display, ...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...difficult to study without biosensors to directly visualize their activity. In 2019, the first generation ...developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019). But this first...

  16. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...labeling; multicolor labeling would enable the visualization of multiple loci. Such a system could then be...major applications to cancer, for example, the visualization of chromosomal translocations or chromosomal...

  17. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...to apply for a position.  A job description is usually a wish list and the hiring manager knows that no... know the exact person monitoring submissions (usually a Human Resources representative). Pro tip: if ...

  18. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...imaging with fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail... lux. arg1 and lux reporters both allow for visualization of the bacteria, but lux imaging shows only ...
Showing: 61 - 80 of 351 results