...and so far most of their funding has come from kickstarter and fundrazr projects). This allows the project... to a wider group of people. This is a perfect starter project for our computer scientists, many of whom...interested in biology but don’t really know how to start applying their skills to it. The Kittybiome project...Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding the financial...find a makerspace somewhere near you. The place to start looking for your local space is the Hackerspace ...

... For anyone just starting to use flow cytometry, it’s generally recommended to start compensating with...needing to use your single-color controls as a starting point. Applying compensation to your experimental...your main population and singlets. Then, you can start to compensate on your experimental samples. Let'...challenging! But we hope this guide can help you get started. Remember, you can always change your compensation...

...they are useful, how they work, and how to get started with them. Why Use multicistronic vectors? Detecting...added to the end, while the downstream cistron will start with the Proline.  The table below lists the four...peptide to improve cleavage efficiency. How do I get started? If you are looking to co-express your gene of ... protein or selectable marker, it is easiest to start with a plasmid that already has the multicistronic...co-expression of multiple unique genes, you can start with a plasmid that has multiple cloning sites flanking...

... retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated...hemophilia, muscular dystrophy)? AAV gene therapy started in the mid-90's with a lot of work and a little... luck A pioneer in the field, Dr. Jean Bennett started working on ways to deliver genes to correct genetic... another 6 years before the 1st clinical trial started, and 10 more years to obtain FDA approval and make... first clinical study combining these tools may start as early as next year. Twenty years after falling...

...kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product: plasmid... DNA gel extraction with glass syringe filters Starting material: bacterial cultures or agarose gel slices...commercial kits.  DNA gel extraction with glass milk Starting material: agarose gel slices Product: DNA fragments... enzymes.  Cellulose nucleic acid purification Starting material: plant, microbe, blood, or mammalian ...Regeneration of silica columns for DNA purification Starting material: PCR product or agarose gel slices Product...

...producing and purifying it in your lab. Instead of starting a new search or clicking through to a different...Materials or elsewhere in their papers. Maybe they started their deposit prior to publication and were able...searching with filters Not every plasmid search will start from a publication. You may have an idea for a plasmid...express ubiquitin under control of a CMV promoter. Starting with two search terms we see there are plenty ...need to apply different filters you do not need to start a new search. Simply click the ‘X’ next to any filter...

...can have when starting in an academic lab. I floundered for a solid year after I started my postdoc! ...cloning and lentivirus production, I didn’t really start to recognize my transferable (or “soft”) skills ...you’re out of the lab to think about these--get started with our Transferable Skills Guide.     Over ...gloves again! The opportunities are there once you start looking for them. Acknowledgements Big thank you...

...suspenseful. 4. Start preparing your talk early - like today Don’t wait until you need to speak to start creating...’s attention will start to wander when you are supposed to be done. They will start looking at their phones...future boss in the room, so it is a good idea to start thinking about public speaking early and often. ...

...field is simply to know where to look and how to start. There is value, then, in having a guide that curates..., podcasts, and communities to help people get started in synthetic biology. While not everything is open-access...various applications. I think these papers are good starting points to understand the contours of each topic...many people will find this guide useful as they start their synthetic biology journey. And if you have...

...just dive right in and start injecting your virus, a successful AAV experiment starts with validation and...your system that can affect expression, before starting the selection process. Factors to think about ...expression.   Once you know that, you’re ready to start selecting, testing, and optimizing three key variables...in your system and then test each vector before starting your experiments (Rolland et al., 2016; Su et ...

...step. As with standard IF, you will need to start with a fixed sample. Depending on the sample type...photobleaching or by stripping out the antibodies, then start over with the next target: add the new primary, ...Abcam's sample protocol is a good place to get started.  One important consideration when deciding whether...not being degraded. Whether you’re just getting started with two or three targets or you’re pushing the...variations you could try. We hope this post helped you start planning for your next multiplex IF experiment! ...

... grad school to a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an...to the UK with foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic...Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael Koeris Resources...

..., we discuss some of the many things you can do start your own science communication student group and... and Amy say is the quickest way to get things started? Set up your own Science by the Pint series and... best way to get a Science Communication group started. It's as simple as getting people together at a...email [email protected]    For more info about starting your own SciComm organization and to learn about...

...frustrated and no closer to starting your experiments. Get organized now! Start by gathering that pile of...or projects) to make it easier to know where to start your search. Then identify the most frequently used...ways to share your documents with your colleagues. Start small by identifying the protocols, guides, or references...

... picking your research topic, starting a lab, and more. Before we start, we'd like to extend a special... "Choosing Your Research Project," “Advice for Starting Your Lab,” “Fun in the Lab!" and more.   Acknowledgments...Choosing a Research Project Advice for Scientists Starting a Lab ...

...expression, is it necessary to add a G nucleotide to the start of my target sequence? A2: The human U6 promoter...promoter prefers a 'G' at the transcription start site to have high expression, so adding this G could help ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...that mutation be selected/screened? A4: Before starting your experiment, consider co-transfecting with...

...agreed to come in and start getting plasmid deposits into the collection and to start shipping. They created...closure was earlier than most companies. Since the start of this crisis, we’ve been very responsive to the...Addgenies home.  At the same time, as Addgene started dealing with this new reality of working remotely...

...alternative” anymore.   Since most scientists start out in academia, it is especially important for ...calendar Academic researchers must be extreme self-starters and especially good at self-promoting—No one is...about something like this without sufficient data? Starting to learn about the diverse options and how to ...Science Careers How to Negotiate a Successful Lab Start Up Budget Read Our Management for Scientists Series...

...their position relative to a transcription start site. In STARR-seq, randomly sheared genomic libraries ...(Q3) MARCH 2016Enhancer activity mapping with STARR-seq   Although many technologies have been developed...sequences in different chromatin states. Alexander Stark and colleagues at the Research Institute of Molecular...Pathology have developed an alternative method, called STARR-seq (self-transcribing active regulatory region ...over input levels using next gen sequencing. The STARR-seq backbone is available from Addgene in both human...report using as few as 150 fly neurons as their starting material! Thus, TRIBE can be used to analyze a...

...government sectors.  Within these sectors, you could start your own company, work in a bench research career...sponsorship. If you are in this position, you should start planning for your visa and or green card process...consulting firms), sponsor more H-1Bs than small startups and firms with a non-international focus. Candidates...applicants received sponsorship) then he or she can start working in October of the same year. The Industry...are not already reviewing papers that you should start contacting journal editors to request to be a reviewer...