We narrowed to 546 results for: TIL

... and put it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult)....diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming with CRISPR/...

...CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global transcriptional ...rescue the neurological defects of Rett syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett... . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J...398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 ...

...2019) If you’ve taken the time to do this and are still struggling with idea generation, try this thought...with an out-of-the-box concept and pare it down until you have something tangible and applicable to your...to the same problem. If your lab/company/team is still working towards a culture of encouraging dissent...easier to work with than nothing at all - they’re still ideas, after all, and that is the goal of this exercise...

... plasmid is delivered to your target cells, you still need to select for the cells that contain it. Antibiotic... approach in a mammalian cell experiment should still include an antibiotic resistance gene for growth...express your protein of interest. However, you do still need a species-compatible promoter for expressing...between prokaryotes and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak...

..., there still remains the problem of prevention. Asbestos exists pretty much everywhere, still, in old...mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the hope is that...United States and many other countries, and it is still imported, exported, and used throughout the world...

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ...University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...

...the Brian Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site ...conducted multiple rounds of directed evolution until they identified a mutant with significantly increased...cells. To circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...

...be visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that...homozygous clones, however, sequence confirmation is still needed to verify the correct sequence was inserted...it requires a little extra wet lab work, this is still a great option, especially if the options outlined...data analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing...

... too many moving parts in the whole process to distill a one-size-fits-all recipe for success. What I ...the Dean, and even some people in your field that still need to understand your specific questions and experiments...directly state your official aims, but I suggest distilling them into simple goals/outcomes. This isn't the...show interest in other faculty members’ research. Still a Grad Student? Get Tips on Getting the Most out...

...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...in-lab filming and remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth...

...repair or add in disease-causing mutations, but, until recently it hadn’t been used to treat disease postnatally...the amount of research conducted on DMD, there is still no good treatment. The dystrophin gene is very large...approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option for...CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized...

...used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, piggyBac™ has ... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,...

...specific agonists for orphan GPCRs demonstrating the utility of this platform for drug discovery. Researchers...CCP); the latter of which greatly enhances the utility of this collection as it can be used to easily ... allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually 30-40bp ... are available at Addgene. Since GMAP assembly utilizes 5 different overlap sites, this technique also... repeats into a single-tube, 3 day process by utilizing pre-assembled trimers to reduce the number of ...appealing tool for studying essential proteins. Until recently, it was challenging to apply the AID-tagging...

...molecules are then activated in the same manner, until enough molecules have been analyzed to allow for...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable... the scientific community, and new variants are still being engineered. By combining PA-FPS with superresolution...Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy...

... or topic of interest. Despite its power and versatility, the technique is remarkably straightforward ...dependent on the promoter of the cre recombinase while still taking advantage of the robust expression via the...best to spend the time practicing your injections until they are accurate and reproducible. I also highly...likely be less than that of viral expression but may still be sufficient for successful stimulation. Testing...

...obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing... to understand how to use it. TLDR: This is a versatile mammalian expression vector that you can use to...transfection by western blot. Overall, this is a versatile mammalian expression vector that you can use to...

...expression, though it is possible for the plasmid to still express without the G. Because the G is only one...the efficiency will be lower, although this might still be okay if you are introducing a selection marker...if/when you use HercII or Kapa Hifi the reaction still does NOT work, you can try these new primers: EMX1...Mukherjee, K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...is a great option because it provides a great versatility on how to use your plasmid in every type of experiment...

...to keep your deposit on hold from public access until the article is published. This policy gives us time... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...

...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...personal growth do not exist or if they feel underutilized in their present positions By removing the...will address more specific tactics managers can utilize to be in touch with the needs of the team and to...