We narrowed to 74 results for: UBC

1. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...

2. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   Published

   Nov. 5, 2014, 3:41 p.m.

   ... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...

4. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

5. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...only includes general lab strains designed for subcloning or protein expression. If you were to include...

6. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

7. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ... as seen with other epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral...

8. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...

9. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

10. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

11. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...

12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

14. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...channel). The Guler lab optimized the actuator’s subcellular localization using trafficking signals and named...