...changes of these Twitch sensor variants were optimized by testing various linker configurations in a large-scale...from muscle cells. They identified a toadfish TnC variant which had a high-affinity for calcium binding and...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat...“trick” to the development of additional color variants. This resulted in the construction of redCALWY...redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor ...

...biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting...serotype testing. These samples are available in various serotypes of two popular control AAV encoding GFP...basal fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large ...-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262  AAV1 pAAV-hSynapsin1-axon-GCaMP6s...Name 105322  AAV1  pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s...

...Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University of Baroda, India  Devarshi Gajjar has 10 years...antibiotic susceptibility of biofilms formed on various structured surfaces. Plasmids expressing fluorescent...astrobiology. During the last 11 years he has studied various aspects extreme life on earth including extremophile...scientists globally, Addgene has partnered with various community organizations and workforce projects ... Various Addgenies...

...be used to study dynamic processes. Conversely, varied input patterns may also be used to engineer new...response regulator (RR). TCS-induced responses are varied; one outcome is directed transcription from a given...obligate chromophore allows the system to respond to various light inputs. In far-red light or dark conditions.... A hybrid histidine kinase/photosensor senses various light inputs, and an obligate chromophore allows...They experimented with the promoter strength of various components, removing inducible promoters that could...many of these systems could be made suitable for various scientific or engineering applications.   References...

...technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied with a luciferase...collection of multicolored nano-lanterns for use in various applications, including optogenetics, biosensors...BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent protein...requires a linear unmixing algorithm to separate the various colors. In fluorescent imaging, you use the excitation... wavelength partially separates the signals of various Nano-lanterns, but it does not isolate each Nano-lantern...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

...uncover new gene variants associated with conditions of interest, then studying those variants in model systems...The goal of Kiran Musunuru's lab in Harvard's Department of Stem Cell and Regenerative Biology is to ...implicated in disease or, in cases where particular variants or mutations have been reported in people, we ...for decades to generate knock-out mice, but for various reasons it is not nearly as efficient in human .... You could use the kit to insert all of those variants into stem cells with the same genetic background...

... can also be attached to an antibody to detect various targets, such as proteins, nucleic acids, or glycans... These consist of a genetically encoded enzyme variant tag that covalently reacts with a small substrate...protein of interest. Fluorescent dyes and ligands vary in their chemical structure, leading to differences...in biotechnology (Grimm & Lavis, 2022). These variations directly impact the way you can utilize these...mitochondria, and different dyes can result in varying cytoplasmic background signals when used with the... that different fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors... ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness, depending on the respective...

...through the path of a laser and interrogated with various visible and fluorescent light sources that allow...and target protein composition. It is used for a variety of applications such as measuring target protein...addition to visible light, flow cytometers can have a variety of fluorescent light sources. When interrogated... detectors. The strength of the emitted photons varies from cell to cell depending on the level of fluorescence...particularly well to complex experiments looking at a variety of different targets. If you are planning on using...Alternatively, they could create additional dot plots of various CD markers to further separate the B cell population...Success in a flow cytometry experiment depends on a variety of factors including sample preparation, staining...

...designed 2 variants of EosFP that can withstand these conditions- mEos4a and mEos4b (12). Both variants are ...20 different varieties of PA-FPs available to the scientific community, and new variants are still being... random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F / N99K ...respectively. When illuminated with violet light, both variants are photoconverted to forms that emit brighter...PA-FPs. Several other irreversible PA-FP spectral variants have been discovered such as Kaede and PS-CFP2...

...light chains) each with a constant region and a variable region. The chains are covalently linked to one...the Y and comprise the entire light chain and a variable and constant region of the heavy chain. As the...structure with important features labeled. V = Variable domain. L = Light chain. C = Constant domain. ... Fv - These fragments are composed of just the variable domains of the heavy and light chains of an antibody...resulting in a product called a single-chain Fragment Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison...Introduction to AntibodiesSingle Chain Fragment Variables (scFvs)Avoiding the Mouse on Mouse Mess in IHC...

...plasmid copy numbers vary within cell populations and over time resulting in variable gene expression that...overrepresented compared to those further away. As a result, variable gene expression can make data hard to interpret... in the same background resulted in a 20-fold variation of gene expression. Because pSC101 is not representative...also tested in different plasmid backbones that varied in size or carried different genes. In these cases... complex, stabilized promoters can eliminate variability between the different components. When your pathway...

...expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold...specific target sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion...Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency...compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...  Various Addgenies...

...recombinant antibody. Researchers first sequenced the variable domains of Myc1-9E10 in the late 1990s (Fuchs,.... As a result, you can now find Myc1-9E10 in a variety of isotypes like rat IgG1, human IgG2, mouse IgE...anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand, might...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds... Myc tag? Hardly! 9E10’s accessibility and the variety of formats you can find it in likely mean it will...Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence...

...blogger Jon Chow, an immunology PhD student at Harvard University. Do you have a gene of interest but ...certain that they are too young to mate. Using various markers (more on that below), you can select for...look like stubble (sb). For a better reference of various fly phenotypes, check out the Classical Genetics...available at Bloomington, but you can search for various fly reagents on the Bloomington website as well...ready to start working with live animals yet, Harvard’s DRSC/TRiP- Functional Genomics Resources website...Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly a decade of experience...

...templates. Cas9 variants PE6e–g often had even higher editing efficiencies but varied depending on the...vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous...PE6d are optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits...edit.   Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and... DIRECTED can increase on-target delivery with various delivery modalities and has the potential to reduce...

...The infectious titer of a viral vector stock can vary based on transduction target and can also be altered...Stoilova-McPhie, Center for Nanoscale Systems, Harvard University.    Which titer do people usually...quantified with a different primer pair. Ct values vary based on the amount of starting material in the ...particle. Finally, the precision of the assay can vary up to two fold for the final titer value, which ... same PCR twice can give titer measurements that vary up to two-fold. How can we get more reliable AAV...expression analysis with low abundant targets: from variable nonsense to publication quality data." Scientific...

...recombine independently, cells may acquire one of a variety of colors when a combination of XFP are expressed...systems, many based on Brainbow, now exist for a variety of species, cell types, and research applications...inversion of two genes aligned head-to-head. Another variation, Brainbow 2.1, uses Cre to trigger the inversion...similar systems have been developed for tracking various cell types and organisms and for improving the ...developed from the Brainbow 1.0-L construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP...labeling systems allow single cell dynamics in a variety of organisms to be observed in more detail than...

...served as the Chief Human Resource Officer for Harvard Medical School. The addition of Cicalese to the...member. When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned... committee because I had recently retired from Harvard, and it let me keep my toe in the water of HR work...understand the science but my prior HR experience at Harvard Medical School means I understand the needs of ...served as the Chief Human Resources Officer for Harvard Medical School where she brought leadership teams...

...Researcher at Harvard Medical School, former dean of the faculty of Medicine at Harvard University 29:...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility... talk and at the minisymposium. You can find a variety of additional resources and information about organizations...Science) Edward J. Hall (Professor of Philosophy at Harvard University) Tony Cijsouw (Neuroscience postdoc ...points out in his talk (1:24 - 29:31), there are a variety of ways of defining scientific reproducibility....

...made it easier to create knockout alleles in a variety of species, including the standard laboratory mouse...see deletions of the region between the gRNAs. A variety of other undesired outcomes, including one loxP...Easi-CRISPR for simple knock-ins and floxed models varies from 8.5-100% of pups born, with typical efficiencies...30-60%. Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example...correctly edited founder. Figure 6: Diagram of ovary and oviduct anatomy with the equipment used for ...female mice using a small incision that exposes the ovaries and oviducts. After solution injection into the...