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We narrowed to 213 results for: Xpa

Showing: 61 - 80 of 213 results
  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse...
  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...for lists of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI...
  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector...with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...
  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...
  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ...
  6. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring...
  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was...
  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor...
  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...after your training. You must ensure that you are expanding your options and preparing for both academic and...conflicts PI has no idea who is dating whom Expanding your skillset Scientists are lifelong learners...
  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ...
  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9...
  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... offers additional PAM options that can greatly expand gRNA options. Created with BioRender.com...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...
  13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...
  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus...
  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering ...protein function and signaling networks continues to expand. The labs of Jesse Rinehart and Dieter Söll at ...
  16. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX...
Showing: 61 - 80 of 213 results