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We narrowed to 319 results for: bre

Showing: 61 - 80 of 319 results

  1. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...you obtained the desired chimeric mice, you would breed them many times, hoping to produce a mouse with ...need to remove the selection marker, likely by breeding to a mouse line with a recombinase. If it turned... insert two loxP sites. Since two double-strand breaks are made, it is common to see deletions of the ...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... is usually accomplished by Cas9-mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous...although this is rarely done). When targeting a dsDNA break for HDR, the choice of target site is far more constrained...which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C>T and A>G base editors...

  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...homology arms of 35-80 bp, and DNA double-strand breaks up to 54 bp from the desired insertion site result...knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination repair pathway...interesting to note that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed throughout...is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's important...(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016)....

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then follow the typical...NK) cells.   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment...the sample through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...al. 2022. Image created with BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory.../ENEURO.0373-21.2022 Brown, D., Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru...Kumar, S. R., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P... Kumar, S., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ...converts a C to U without producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com...

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...other organisms, CRISPR-induced double stranded breaks are lethal because the non-homologous end-joining...ineffective in bacteria, CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini...genomic locations, and introduces double strand breaks, the bacteria are unable to resolve them, and the...

  9. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...talk to scientists from around the world making breakthrough discoveries!” I knew right then I had found .... Summaries of the latest technologies and breakthrough research. Insights about how to navigate through...

  10. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom...

  12. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments into its ...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... five strains of Ebola representing the latest outbreak and earlier ones, along with links to the relevant...content. -And the latest updates on the ongoing outbreak from the CDC and WHO. - For anyone interested ...

  15. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...most CRISPR systems and generates double strange breaks at target sites 5' of the 5'NGG3' PAM sequence ...which nicks DNA rather than creating double stand breaks and may better facilitate homology directed repair...

  16. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into...shared, every collaboration formed, and every breakthrough enabled brings us closer to making an impact...

  17. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from customers...favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  18. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...accomplishing it all. How do we encourage people to take a break from their everyday tasks and make time for special...However, I think it’s just as important to take a break from work to share time with the people around us...

  19. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...

  20. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...through normal mechanisms. It will take the full breadth of our research expertise, but we believe that ...health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the team from the...
Showing: 61 - 80 of 319 results