We narrowed to 137 results for: ckb

1. Inside Addgene's 10 Year Anniversary Gala

   Type

   Blog Post

   Published

   July 3, 2014, 3:48 p.m.

   ...thing was clear: the sense of family is still the backbone [ba-dum-chh] of this crazy group of Addgenies....

2. Mapping the 4D nucleome with CRISPR/Cas9

   Type

   Blog Post

   Published

   Aug. 11, 2015, 2:30 p.m.

   ...Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Cell. 2013 Dec...

3. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even changes the DNA sequence...

4. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   Published

   Feb. 1, 2018, 3:07 p.m.

   ...information contributed by the depositor, published backbone sequences, and any known sequences for the insert...

5. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...on Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find ...

6. New CRISPR Web Resources and #12DaysofCRISPR Recap

   Type

   Blog Post

   Published

   Dec. 12, 2017, 3:13 p.m.

   ...explains why pC016 - LwCas13a guide expression backbone with U6 promoter has already earned a blue flame...

7. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   Published

   June 30, 2015, 2 p.m.

   ...flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid contains the transposase gene...

8. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

9. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...prokaryotes to deliver the technological biological blockbuster known as the CRISPR (clustered regularly interspaced...

10. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1,...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...pBLADE-mCherry: This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry...

12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful...

13. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...the pair comes from a topoisomerase I-linearized backbone. DNA topoisomerase I (depicted as a green cloud...

14. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

15. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    Published

    April 7, 2022, 1:15 p.m.

    ...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ...

16. Hot Plasmids - January 2023

    Type

    Blog Post

    Published

    Jan. 31, 2023, 2:15 p.m.

    ...and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et ...

17. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with...

18. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...

19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

20. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags  ...