We narrowed to 317 results for: cript

...create secondary structures in RNA transcripts Effects on transcription (remember, it’s not all about translation...locations or fail to make it through the entire transcript resulting in the production of nonfunctional ...translation - e.g. codon choice may interrupt transcription factor binding sites) As you might imagine,...algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization tools. Keep in ...

...Find the anti-CRISPR plasmids Tagging human transcription factors with GFP Kevin White’s lab recently ...deposited a set of CRISPR plasmids to tag human transcription factors with GFP. As part of the Encyclopedia...these donor plasmids will help researchers map transcription factor localization and binding via ChiP-seq...ChiP-seq. Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR...

...the submitted application, along with a brief description of the experiment. If that data was included ...ELISA — but in all cases, it contains a detailed description of the experimental conditions.     Figure 4... the conditions described above, and a short description of how the antibody performed.  Figure 5: Results...The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate, and...

...tools in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly...site to cleave and inactivate any read-through transcripts of the effector gene. Effector genes, any gene...across cell types and organisms by replacing the transcription factor binding site and effector in the construct... the SPARC system to use other experimental transcription activation systems, such as LexA/p65 or mCD8...

...more detailed level, the Gal4/UAS system is a transcription activation system co-opted from yeast (1). It...workhorse to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences...sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus...Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703...

...responses are varied; one outcome is directed transcription from a given promoter. To harness the power ...the RR (OmpR) via phosphorylation, promoting transcription (see figure below). Subsequent exposure to red...response regulator via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red...types of proteins, including Cas9 and dCas9-transcription factors, for which low expression levels are...

...example, IZKF1 and IZKF3 are cell-type specific transcription factors with important roles in Multiple Myeloma... (Nabet et al., Erb et al., Huang et al.), transcriptional and epigenomic profiling studies (Nabet et ... 29581585. This reference contains the full description of the dTAG system. The dTAG system was employed...Featured molecules: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia...

...and IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...changes in RC2H1 protein expression and post-transcriptional regulation of SM1v1-containing RNAs could play...IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound by IRE binding proteins...

...ligands NRs represent the largest family of transcription factors found in metazoans. The superfamily ...profiling datasets in to our data mining tool, Transcriptomine. We are currently nearing the completion of...transient transfection platform for assaying transcriptional activity, for example, was a key development...coregulators: Judges, juries and executioners of transcriptional regulation. Molecular Cell 27, 691-700. (PubMed...

...on a fusion enzyme of Cas9 nickase + reverse transcriptase and a prime editing guide RNA (pegRNA). The ...) complementary to the genome and a reverse transcriptase (RT) template including the desired edit. ...., 2019). Test different lengths of reverse transcriptase template, starting with about 10–16 nucleotides...pegRNA primer binding site (PBS) and reverse transcriptase template (RTT). Created with BioRender.com. ...

...how much data is necessary before submitting a manuscript. In fact, how many of you have negotiated over...over the order of authorship for a manuscript? And that’s only in your scientific life! I’m certain that...mate would have time to make comments on your manuscript. If you give her this slot, she’ll have extra...her deadline) to help you. Her feedback on your manuscript is more important to you than collecting your...

...C-to-U and A-to-I transcriptome-wide off target effects observed in whole-transcriptome RNA-sequencing, ...amount of off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target...the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency. They note...

...enables us to quickly screen and investigate transcription factor candidates and the regulation of genes...technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver...use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test...Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International journal...

...in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp ...recently created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion... HaloTag dyes and showed they could record transcriptional activation events as bands of induced GFP-iPAK4...Analyzing the ticker tape images could recover transcriptional histories, accurate to within 40 minutes. ...

...quantify RNA transcription by RNA Polymerase III. By expressing Corn under promoters for transcripts uniquely...tagging RNA sequences with an aptamer and RNA transcription by placing the aptamer downstream of a known...Wenjiao, et al. "Imaging RNA polymerase III transcription using a photostable RNA–fluorophore complex....

...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the...3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from a designed repeat protein...the production of a downstream peptide, the transcription terminators in this series are just the tool...

...therapeutics - one using dCas9-SAM to activate HIV-1 transcription and destroy infected cells, the other using ...histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV-1 genome. For a targeted...dCas9-VP64 fusion commonly used to activate transcription, but with two additional activation domains ...domains (MS2 and p65) to enhance transcriptional activation. Zhang et al. designed multiple gRNAs to target ...

... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-PHP.eB, when paired with ...target gene expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested ...when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its tropism is relatively more biased...Characterization of AAV Tropism and AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers...Wang, A. Z., & Gradinaru, V. (2023). Spatial transcriptomics for profiling the tropism of viral vectors ...

...epitope-tagging experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this...epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl .../index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA...strand (strand used by RNA polymerase II for transcription) are more effective for genome-editing events...

...community is generating a massive amount of descriptive transcriptomics data. Custom CRISPR screens designed ...pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter then showed...videos and text on the website for more detailed descriptions of how to use the software. Here is a brief ...