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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 214 results for: gnal

Showing: 61 - 80 of 214 results

  1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...internal and external signals such as developmental cues and DNA damage. These signals activate a cascade... however have several limitations. First, FRET signals are often difficult to detect, and thus quantify...

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... increased brightness, they also found that the signal to noise ratio can be increased from 2-fold (by.... Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, they chose...Hanhui et al. “CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.” Nature Methods...

  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...assays have lower background levels and improved signal sensitivity. Another advantage: luciferase reporters... Cofactors/substrates Required Not required Signal strength Lower Higher Sensitivity Higher Lower... similar approach can be used to analyze cell signaling pathways or miRNA knockdown. BRET for the best...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...various labels to antibodies that produce detectable signals like light or color. In this post, we’ll go over...is not visible or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin... molecule. These properties allow for efficient signal amplification, making biotin conjugated antibodies...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled...scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA* was tested...timescale, such as during drug action or cell signaling. Limitations of RaPID Like other BirA*-based proximity...

  6. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...polyclonal, as their function is to amplify the signal from a primary antibody; the ability of polyclonal...their target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal...positive control across experiments to be aware of signal strength. In general, polyclonal antibodies produce...

  7. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks, a new...2021). For example, inserting a polyadenylation signal downstream of the transcription start site will... insertion of a synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...rounds of stripping and reprobing, maximize your signal by starting with the least-abundant protein and...your fluorescent probe. Then, if you don’t see any signal, you’ll want to check for the primary antibody ...that you’re looking for a “blank” result! If some signal remains, you may want to repeat your stripping ...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...FRET signal. Subsequent demethylation separates the fluorescent units and so lowers the FRET signal back...a large role in turning genes ‘off’ and ‘on’, signaling which genes should be ‘read’ by the cellular machinery...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...mark pixels used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance...profiling or interrogation of context-dependent signaling in primary cells. The authors used this platform...

  11. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...fluorophore. For example, in figure 1, the FITC signal is spilling over into the primary detector for ...are a convenient tool to obtain a strong positive signal for a given fluorophore, as they contain a mixture...convenient in that they provide a strong positive signal, even for rare cellular markers, beads can sometimes...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional resources on...90746-4 Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell...

  13. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...is continuously detected via a fluorescent signal. The signal increases proportionally to the amount of...concentration of the starting template DNA the fluorescent signal throughout the reaction is compared to a standard...

  14. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...identification of signaling pathways with potential to confer anticancer drug resistance." Science signaling 7.357...Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its major appeal is that it enables the...

  15. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...immunodetection tools is their strong signal strength. This signal strength is due in large part to their... be labeled with up to 3 dyes, yielding similar signal strength to a standard IgG secondary antibody (...

  16. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...proteins, promoters, enhancers, polyadenylation signals, inducible gene-expression sequences, and more....promoter (to drive high-level expression) and a polyA signal (to terminate transcription) and the four constructs... containing multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...mScarlet-I3 also offered clearer signals due to reduced background signal when tested in more highly autofluorescent...external light source, they have lower background signal and can be used in light-sensitive systems. Most...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...new improvements. The new variant has increased signal-to-noise ratio and better ability to record subthreshold...they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that... SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques can be problematic...fluorophore and subsequently leading to a positive signal. The same effect can be achieved for a fluorophore...than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...

  20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...
Showing: 61 - 80 of 214 results