Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 148 results for: lenti

Showing: 61 - 80 of 148 results

  1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...(Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing a gRNA. These transduced stem...systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The four systems...pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation CHIME ...

  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents Addgene...establish consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from the University...the Addgene Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...available from Addgene. These plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged...unclear which terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing...multiple FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that targets human glycosyltransferase...inhibition pooled library is now available as lentiviral preps in addition to the pooled library format...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A...-P2A P2A Insect pUltra P2A and T2A Lentiviral Ac5-STABLE2-neo T2A Insect   For the co-expression....) PubMed. 3. Highly Efficient Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et...

  9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Brunello CRISPRko sgRNA library as pooled library or lentiviral prep! Dolcetto: human CRISPRi sgRNA library While...the Dolcetto library! Now also available as a lentiviral prep. Calabrese: human CRISPRa sgRNA library ...function. Find the Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench...

  10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same ...same family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...depression. The researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus...

  14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...

  15. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...

  16. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...

  17. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  19. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... from other researchers, and cloned them into lentiviral vectors to generate a kit which is now available... cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode ...

  20. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...weeks to work on cloning the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked...a 100% successful clone of the Syntaxin3 in the pLenti backbone! Once everything was confirmed, I worked...
Showing: 61 - 80 of 148 results