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Addgene
Showing: 61 - 80 of 225 results

  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... bit of Addgene history... As you may be aware, Melina Fan, her brother Ken Fan, and her husband Benjie...sometimes fun needs to be had in an orderly and timely fashion. 9. Ask for help My grandma used the old...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas... Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides...

  4. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...achievable tasks, and then executing on them in a timely fashion while allowing for creative solutions to...part of the profession, and that they can be ameliorated by thoughtful planning and engagement, leaving...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is a sucrase...Biotechniques 40.3 (2006): 355-364. PubMed PMID: 16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...partner Rho GTPase, leading to behaviors like lamellipodia or filopodia formation, cell contraction, or...they described tools to control formation of lamellipodia and contractile stress fibers, and recently ...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982) combined...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not sequence all DSBs, may miss unpredicted...Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...achieve your intended next career stage and create a timeline for achieving these goals. Planning is often more...not exist to answer the question or it might be extremely difficult for you to get the needed tools and/...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...the variable heavy chain fragment of heavy chain camelid antibodies (Hcab) (Arbabi-Ghahroudi et al., 2017...collection! References: Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective...

  12. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...through promoter-directed expression alone is extremely limited. Therefore, highly expressing, generic... privately communicated to me as one of these extremely high expressing promoters). Conduct a time course...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020). Directed evolution of adenine base...Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. M. (2023). Improved cytosine...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...

  17. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...

  20. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...will be removed before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under...
Showing: 61 - 80 of 225 results