We narrowed to 71 results for: pcas

1. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...three different plasmids to produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep...

2. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing replication factors and serotype-specific...

3. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog...

4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...is more stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific...

5. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...binding domain (LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained...

7. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...also fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility,...

8. Typing CRISPR Systems

   Type

   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ...There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A...

9. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...PE-CF594PE-Cy5PE-Cy7       Red (640 nm)   APCAlexa Fluor 647   Alexa Fluor 700APC-H7APC-Cy7 ...

10. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ... a Cas9-containing destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available...

11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing...