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We narrowed to 23 results for: pcas

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Showing: 1 - 20 of 23 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The vector can ... 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...codon-optimized SpCas9 plasmids can be ordered via the links below: 42230 : PX330; SpCas9 and single guide...guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide...RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase...

  2. CRISPR Guide

    Type
    Collection
    ...pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3'...NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3...engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular...no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) — weaken interactions between the HNH/RuvC...RuvC groove and the non-target DNA strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone...your desired genes for modification. Engineered SpCas9 enzymes with altered PAM specificities are available...

  3. CRISPR References and Information

    Type
    Collection
    ... donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes. Developed ...Design DeepSpCas9 (Link opens in a new window) A webtool that uses deep learning models for SpCas9 gRNA ...pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF, 129 KB Marraffini Bacteria: pCRISPR...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles... for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder Assembly...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN 2.0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination...mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-...robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available...assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian Expression mCerulean 433 475 27 ... dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian ...530 68 5.9 Monomer pJL-mGold - Yeast Expression pCaggs-mGold - Mammalian Expression mGold2s 517 529 90...Bacterial Expression pJL-mGold2s - Yeast Expression pCaggs-mGold2s - Mammalian Expression mGold2t 515 527 ...Bacterial Expression pJL-mGold2t - Yeast Expression pCaggs-mGold2t - Mammalian Expression mCitrine 516 529... Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian... - Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian...

  6. Lentiviral Prep Service

    Type
    Collection
    ...-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting 19,114 human...non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs targeting 19,114 human genes...non-targeting controls. This backbone does not contain SpCas9. 78,637 unique sgRNAs targeting 19,674 mouse genes...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ataxia Bruce Tempel 30137 pCAX APP 695 APP CAG Alzheimer's Dennis Selkoe 30138 pCAX APP 751 APP CAG Alzheimer's...Dennis Selkoe 30143 pCAX APP delta CT APP CAG Alzheimer's Dennis Selkoe 30144 pCAX APP AENATA APP CAG ...Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP C99 APP CAG Alzheimer's...Dennis Selkoe 30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP...-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS...APP CAG Alzheimer's Dennis Selkoe 30154 pCAX FLAG APP APP Flag CAG Alzheimer's Dennis Selkoe 31255 pGEX...pEGFP-C9orf72 C9orf72 GFP CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE...Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-1-PV0102 ...Control Karl Deisseroth AV-2-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-2-PV0101 ...Control Karl Deisseroth AV-9-ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-9-PV0101 ...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target...Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to target every... Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ... AAVS1 genomic safe harbor locus using untagged SpCas9 (Addgene 41815 or #44719 ) in combination with ...using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which ... integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol...

  11. Retrovirus Plasmids

    Type
    Collection
    ...-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid. Arthur... Arthur Nienhuis and Patrick Salmon 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis...

  12. Arf GTPase Family

    Type
    Collection
    ... Mammalian (pcDNA4c, pCAG), Gateway GEF Arfgef2 (BIG2) 10564 1785 Mammalian (pCAG), Gateway GEF Arfgef3...

  13. Lentivirus Plasmids

    Type
    Collection
    ...packaging construct encoding tat . Jakob Reiser 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid. Arthur...packaging construct encoding tat . Jakob Reiser 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...with CMV promoter Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of the Tet-On 3G transactivator...viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the CAG promoter...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Membranes Palmitoylation EGFP Connie Cepko 14757 pCAG-mGFP Membranes Palmitoylation sequence from GAP43...Actin filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin filaments beta-Actin EGFP Ryohei...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2 HSP90AB1 heat...VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine...

  17. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...guanosine, the result is an A to G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

  19. Plant Plasmids and Resources

    Type
    Collection
    ...constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR Sylvestre Marillonnet...

  20. Control AAV Preps

    Type
    Collection
    ...dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8...
Showing: 1 - 20 of 23 results