We narrowed to 329 results for: pho

...Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to create enzymes that...turned the enzymes back on. Xin termed these photoswitchable enzymes Fluorescent Light-inducible Proteins...various designs, Xin has been able to develop photoswitchable Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5,... You can learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in ... the production of HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in ...Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent Proteins Use Light to Control ...

...sensor’s photophysical properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum...signals upon sensing glutamate using both 1-photon and 2-photon imaging.   Figure 1: 1P and 2P excitation...membrane trafficking and localization. Improved photophysical properties The improved variants of iGluSnFR3...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...

...envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for...of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell... produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables additional...

... efficiency by which absorbed photons are converted into emitted photons. These are indicated by the extinction...this series of posts are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness...high brightness is an important feature of a fluorophore. The key question is how the brightness is best...Theoretical brightness Two key properties of fluorophores that determine brightness are the extent to ...Without further information about properties like photostability or aggregation tendency, our choice would be...

... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...non-natural amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability...Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores...better-dyeing-through-chemistry-small-molecule-fluorophores  Los, G. V., Encell, L. P., McDougall, M. G....

...Here's what you'll find in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery...synaptic connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy... current yellow fluorescent proteins tend to photobleach rapidly, which hinders their use for long-term... The mGold2 variants significantly enhanced photostability — 25-fold over mVenus and 4-fold over the previous...mGold2s, or mGold2t. Bottom: Quantification of photostability. Image reused from Lee & Lai et al. (2025) ... Lee, J., Lai, S., et al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging...

...clue 1 below!) You will find five photos this way. At the very last photo, you will find a link to a form...workspace over the last year. Now, we’ve collected photos of our pets with Addgene gear, and have unleashed...Addgene-branded clothing on the blog. Below each photo, the pet gives you a clue to help you find the next... next picture. If you find the next correct photo, you will see a reference to this scavenger hunt and...

...more! Read on to learn more about: Highly photostable mScarlet3 variants Addgene's own AAV2 and AAV5...brightest red fluorescent protein, but its modest photostability limits applications like long-term imaging ...mutagenesis and screening strategy to select photostable variants, resulting in mScarlet3-S2 (M163H/M66Q...mScarlet3-H showed up to 29- and 15-fold higher photostability than mScarlet3, respectively. Although they...comparable or a bit better than mCherry), their photostability means they more than make up for it over the...Wang, K., Xue, F., … Xu, P. (2026). A highly photostable monomeric red fluorescent protein for dual-color...

...of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) ...) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically. Fortunately...from your source and to your camera.  Two-photon image of miRFP670nano3-expressing neurons in the... derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness than...were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019). They were engineered...Molecular brightness vs. iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells...

...et al., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve...expect to see systems that use newer, more photostable fluorophores and that can be combined with new imaging...their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered...clearing methods and can be labeled by synthetic fluorophores, which are more stable than fluorescent proteins...

...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

...last year, Verkhusha added two far-red light photoactivatable (PA) near-infrared fluorescent proteins (FPs...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...genetically-encoded probes by making them brighter, more photostable, smaller, and less toxic to cells. They’d like...751-4. doi:10.1038/nmeth.2521) Far-red light photoactivatable near-infrared fluorescent proteins engineered...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

...for up to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents... of mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication...10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination...

...signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted...Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein...

...SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore (circularly...three-dimensional cultures that mimic aspects of organ morphology and physiology in vitro and have emerged as a...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...

...migration of a cancer cell line equipped with photodissociable EB1 could be stopped in areas with blue light...manipulating and studying cellular dynamics. The photoinducible control of protein function is more specific...signal. Prior to the development of these probes, fluorophore binding aptamers and MS2-binding fluorescent ...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...

...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent... intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation,...reversible and do not cause structural deformation or photo-induced damage. These fusions can also function ...

...fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...useful in multicolor labeling particularly with fluorophores that excite in the violet range. For example...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

...sfCherry2 sfCherry2(1–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split...iRFP713. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared...domain. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric...typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid and reversible...flexibility in color labeling with exogenous fluorophores, which can even be bright enough for single-...

... molecules, which are much brighter and more photostable than fluorescent proteins, making them ideal ...versions. ASAP5 can be imaged using one- or two-photon microscopy to detect action potentials and subthreshold...fluctuations in awake behaving mice through two-photon microscopy at depths up to 600 µm. Figure...recognizes the disease-associated version of Tau (phospho-Tau). We hope these will become valuable tools ...