We narrowed to 106 results for: sacs

1. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

2. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ... crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 ...

3. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major incentive to use cold-active...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...’s Association (HBA) Boston Chapter and the Massachusetts Association for Women in Science (AWIS) mentoring...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...or hPR ligand binding domains and the MSN2 transactivation domain. Transcription factor activation via...

6. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...non-renewable reagent - Their production requires animal sacrifice and because they are an animal derived product...

7. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

8. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method...

9. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

11. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

12. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

13. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting...

16. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture away from the comfort of...

17. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133...

18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...scale right now is to grow up a bunch of cells, sacrifice them all, blow them up, and slam that onto a mass...

20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020...