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Addgene
Showing: 61 - 80 of 106 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...activator rtTA (reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline...

  2. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

  7. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...non-renewable reagent - Their production requires animal sacrifice and because they are an animal derived product...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library - Ampl133...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture away from the comfort of...

  20. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098...
Showing: 61 - 80 of 106 results