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Addgene

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We narrowed to 286 results for: tac

Showing: 61 - 80 of 286 results

  2. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...with the main character in the video. We’ve grown attached to the work, and I confess that, when I finish...of our inner children who have no preconceived obstacles, optimistically grasp at limitless potential, ...

  5. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly... ours reflect a growing effort to address this obstacle in its many forms. A short stroll from my office...

  6. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...nothing if not solving your way through a series of obstacles in pursuit of achieving knowledge. Or, as we in...result of competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent...

  7. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands...topoisomerase I from rebinding, which results in more intact molecules. (Note that the amount of salt you add...

  9. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...with a valid email address).  One may also simply attach important references that serve to further characterize...much appreciate feedback, so try out the site and contact us if you have any ideas or requests.  Or, if you...

  11. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...

  13. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...would like to try and meet. Don’t worry about contacting someone much more senior than you – meeting scientists... level of training is always a good idea. Make contact with other attendees in advance. Join a LinkedIn...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... lysis, RNA is degraded. To effectively isolate intact RNA, a fast, strong protein denaturant would be...ultracentrifuged for up to 20 hours to separate the intact RNA from DNA and proteins. Although very effective...

  15. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ... look at their profile. Make your professional contact information available. When I am speaking at a ...touch if you have business or follow up. Use the "Contact info" link right below their photo. 2) Personalize...

  17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...

  18. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...

  19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...

  20. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...
Showing: 61 - 80 of 286 results