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Showing: 61 - 80 of 127 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... reactions are performed, the next step is to transform competent E. coli cells and select the positive...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and the ability to easily...Clone desired DNA cargo into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen...

  5. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...Sikora’s lab Matthew Sikora’s lab went all out to transform their lab into a walking tour of Buddy the Elf...

  6. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... also recently described how the glycoprotein transforms itself to escape vesicles within host cells and...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... plasmid. The ssDNA template is generated by transforming a backbone phagemid vector into an E. coli strain...to remove residual starting template. After transformation and harvest, the library is ready for use. ...

  9. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...scientific community, as they allow for simplified transformation processes, and can be used by scientists across...Plasmids 101: What is a Plasmid?  Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Tranfection...

  12. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team...

  13. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...For those who do want to apply LITEs for the transformation of neurons, she offers a tip: save yourself...

  16. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection...

  20. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...the targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the ...
Showing: 61 - 80 of 127 results