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We narrowed to 7 results for: transformation

Showing: 1 - 7 of 7 results
  1. Molecular Cloning Techniques

    Type
    Guide
    ... with four nicks that bacteria repair after transformation. LIC does not require site-specific recombination... is rendered unnecessary. The product may be transformed directly into E. coli , where the normal replication... recombination abilities of yeast. By simply transforming into yeast two (or more) fragments of dsDNA ... seamless manner. To accomplish this, you can transform into yeast the fragments of DNA to be fused along...
  2. Modular Cloning Guide

    Type
    Guide
    ...E. coli to maintain the final plasmid after transformation. Figure 1: Overview of the MoClo Toolkit components...multigene constructs. MoClo Plant Parts III: Transformation & Genome Engineering Kit Plant Expression, ...plasmids with multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit Plant Expression, CRISPR...Daniel Voytas 76 plasmids for assembling plant transformation vectors for overexpression, gene editing, functional...
  3. Adenovirus Guide

    Type
    Guide
    ...restriction enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene #16399) , which... adenoviral backbone plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy and the...into eukaryotic cells using non-viral methods. Transformation The process where bacteria take up DNA from...
  4. Educational Resources

    Type
    Guide
    ...virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol...
  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... into eukaryotic cells using viral methods. Transformation The process where bacteria take up DNA from...
  6. Lentiviral Vector Guide

    Type
    Guide
    ... into eukaryotic cells using viral methods. Transformation The process where bacteria take up DNA from...
Showing: 1 - 7 of 7 results