...nicks that are repaired by the bacteria after transformation. LIC does not require site-specific recombination... is rendered unnecessary. The product may be transformed directly into E. coli , where the nicks will ...disadvantage is that you need to be set up to grow, transform and purify DNA from yeast. Back to Top...

...restriction enzyme PmeI. This construct is then transformed into AdEasier-1 cells , which are BJ5183 E. coli... into the adenoviral plasmid. One can also co-transform standard BJ5183 with supercoiled pAdEasy™ and ...

...virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating Bacterial Glycerol...