Molecular Cloning Techniques
Type
Guide
... with four nicks that bacteria repair after transformation. LIC does not require site-specific recombination... is rendered unnecessary. The product may be transformed directly into E. coli , where the normal replication... recombination abilities of yeast. By simply transforming into yeast two (or more) fragments of dsDNA ... seamless manner. To accomplish this, you can transform into yeast the fragments of DNA to be fused along...