Tips for arabidopsis transformation
Type
Blog Post
Published
Oct. 25, 2018, 1:23 p.m.
... dip transformation method. The general steps for this method include:
Cloning and transforming a plasmid...obtain optimum transformation efficiency. It can take 6-8 weeks from the date of transformation to having ...2013).
Preparing plants for transformation
Whatever plants you are transforming into will be your T0 generation... ready to transform when they begin flowering. It is important to perform the transformation not long ...pods), your plants may be too old to transform. This transformation could still work, but the efficiency... more chances to recover a transformant.
Use young plants: If you transform early, your plants will still...plants per construct I transform. More seeds mean more chances to recover a transformant.
Remove mature siliques...