We narrowed to 904 results for: CAN

...about being a writer that can be a very painful process. And certain stories can make you feel like ‘I’m...shared with a postdoc, and I’m like, “Oh my god, what can I do?! This is a mistake! Why did I even come [to...that’s something that good storytellers in science can do.” Susan’s number one piece of advice for getting...communication: “Find as many opportunities as you can to get your name on things.” Susan personally found...series, but also being my first interview ever! You can follow her on Twitter @sejkeown. If you enjoyed this...Susan’s NEI internship. Application information can be found here Find tips for using social media as ... staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science communication...

... Always wear gloves, as the RNases on your hands can degrade RNA. Keep a clean work area, which may include...to remove residual DEPC. Alternatively, glassware can be baked at 180°C for at least 4 hours.   If your...layer. The top aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced...samples cold until homogenization and cell lysis. This can be challenging depending on your laboratory situation...TRIzol® and ammonium sulfate stabilizing solutions can be made in house.   A common problem with no-kit ... extraction methods is the carryover of DNA that can potentially complicate results of a downstream application...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful...

...we learned how the Cre-loxP recombination system can be used to induce site-specific recombination events...flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental needs. ...and lox511 are recognized by Cre but lox511 sites can only recombine with other lox511 sites, not with ...Once the SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites ... single gene deleted in your knockout mouse, you can design a FLEx switch conditional knock-in cassette...rescue experiment with a wildtype copy of a gene, you can also design FLEx switches to conditionally test the...orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest...

...need. You can find all the hot plasmids from 2017 below. With over 50,000 plasmids, we can't write posts... a valuable tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons...control of enzymatic activity. In addition, proteins can be “caged” by fusion to specific domains thereby ...mCherry variants to the repository. These variants can be used as tools to learn about the influence of ...interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain...detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure S12 of the publication... ladders (aka DNA molecular weight markers) that can be used to measure the size of DNA fragments. Depending...

...License. Importantly, even commercial antibodies can be tethered on the viral surface, expanding the range... with fusogens from different viral families and can be used to target multiple delivery systems, such...lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...cells directly within the body. Overall, DIRECTED can increase on-target delivery with various delivery...labeling and TransitID By Mike Lacy Proximity labeling can identify protein interactions or map a local proteome...different cellular compartments. They showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label... slow-growing protein fiber in the cytoplasm and can be fluorescently labeled. Using lentiviral vectors...

...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs... division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment...transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated cells will have time...plasmid and its associated resistance gene. They can grow proximal to a colony that does contain the plasmid... benefit it offers is marginal. Fortunately, amp can easily be replaced with carb if satellite colonies...compared to streptomycin. Spectinomycin/streptomycin can be successfully used for plant and bacterial culture...genes in their genomes. If you need a plasmid that can go between plants and bacteria, it’s a great option...

...with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving cells...available sister chromatid, though exogenous DNA can be used as well. Below are some considerations for...donors (ssODNs) with homology arms as short as 40 bps can efficiently knock-in a few hundred bps of sequence...guide recognition sequence in the donor DNA, you can guarantee there won’t be re-targeting after HDR. ...tilt the scales in your favor for an HDR edit, you can try one or more of the below suggestions. Disable... those cells! To take this one step further, you can inhibit cell cycle regulatory proteins responsible...are arrested too long. Prolonged cell cycle arrest can lead to apoptosis in as little as 10 hours, so read...

...response to an experimental treatment can be monitored. This can reveal previously unknown functions for... specific tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to...mutagenesis screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those...abnormal cell types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes...neuroscience investigations. For example, neuronal activity can be tracked in whole brains of swimming, actively ...manipulation of brain activity: optogenetic approaches can be used to determine the consequences of activating...Lineage tracing studies highlight how these advantages can translate into insights in developmental biology,...

...parents who can answer some of the surprisingly technical questions elementary students can ask. It doesn... could contribute to the school, too. Though you can recruit parents from any background, it really helps... our math tables!  Be realistic about what you can teach  5-10 year olds in a single evening. Grades...STEM activity fun,” but rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 ...teachers and other science communicators.  What can CRISPR do? I spent more time on this section of the...way to make this section more interactive. If you can think of a good analogy that involves student participation...engineering technology is moving along much faster than it can be officially regulated. I did not try to sugarcoat...

...and sgRNAs can be found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered...However, using CRISPRa to target individual genes can be relatively laborious because multiple plasmids...expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...distinct transcriptional start site. Because CRISPRa can be targeted anywhere in the genome, it is relatively...BDNF protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out...The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening strategies...used by the Qi Lab for this particular application can be found here.  Why CRISPRa? Although cDNA overexpression...