We narrowed to 803 results for: Cre

... sequences Restriction sites Sequences prone to create secondary structures in RNA transcripts Effects...factors on their own. Luckily, many researchers have created codon optimization algorithms and DNA synthesis...abundance tRNAs in your expression host and thereby increase their abundance. For example, the commercial Rosetta...system for many different genes without having to create new constructs. However, due to problems such as...

...inefficient, and often not reproducible. Prior to creating transgenic cultures or plants, our transient expression... expression method enables us to quickly screen and investigate transcription factor candidates and the...with the MoClo system you can easily and quickly create customized vectors. For more information on MoClo...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...

...s clinical and commercial future, and yet are incredibly difficult to find solutions to. There are many...on them in a timely fashion while allowing for creative solutions to emerge. They enjoy the fact that ...home and at the office. Consulting firms are increasingly aware of the toll that long working hours and...career paths available to MDs and PhDs, and an increasing recognition of the value graduates bring to these...

... table below lists plasmids that can be used to create your choice of fluorescent fusion protein with ...quenching and the fluorescence of the donor is increased. This method is straightforward and quantitative...days and good “gigs” ahead...No offense to BRET, CRET and other RET techniques which have never outclassed... BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    ...

...21–26). Increased scientific funding (17). Federal funding for science has been decreasing for the last... government and, consequently, politics. More concretely, scientists gather information about reality ...institutions, while many also seek to honor and credit women, immigrants, and minorities who have had ...

...most unique model organisms, recognized for its incredible regeneration abilities. The axolotl has the ability..., 2019). To study regeneration scientists have created molecular biology tools to generate transgenic ...Xenopus genes and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

... an organization gets, and creating new avenues of communication can create as many problems as it can...guidance X and Y might be able to work together to create solution C. And if a conflict resolver does their...have learned that by working together, they can create unique solutions that benefit themselves and their...

...complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound...label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either the...or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan...588–600. PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled...

...phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS) for the ...residues in the above study with phosphoserine (to create a pSer/phosphomimetic dual mutant) elevated kinase... as an alternative to phosphomimetic mutation. Creating phosphoserine-incorporated proteins The generation... This is a particularly important control when creating a phosphoserine-incorporation mutant for a residue...

...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...