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We narrowed to 790 results for: Cre

Showing: 391 - 400 of 790 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...be cloned into E. coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished... repair sheared ends with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested ...construction for functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 ...Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology...

  3. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...IWGAV five proposed antibody validation pillars. Created with BioRender.com.     Orthogonal Strategies...knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...opposite of the Genetic strategy and looks for increased antibody signal in samples that have had the target... target protein expression experimentally increased. The simplest version would be to use cells that do...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...individuals wherever the cell cultures are kept will increase the risk of contamination. Mycoplasmas can spread...even though the viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the... not be stored in a single incubator since it increases the risk of cross-contamination. CO2 incubators...Elimination is a very time-consuming process and increases the risk of secondary contamination to other healthy...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30...character revolts against class divide that is created by genetics.    Zinc finger nucleases Zinc finger... it can read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly...space travels. The movie sees the USS Enterprise crew fight against a genetically engineered superhuman...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...more photons are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   ...brightness levels on your smartphone or computer screen), and you should be able to properly label many...included—the live/dead stain. This stain is in fact not created through the actual APC-Cy7 fluorophore but displays...absorbed and emitted by matter, and how this process creates visible colors. Next, you learned about how fluorophores...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...public. Find out more at www.projbridge.com. Walnut Creek, CA, USA 37.9101°N 122.0652°W – Steph Hays When...microbial interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321...others come out who feel the same.  I was also incredibly impressed with how well organized the march was...was, as well as the friendly atmosphere that was created by the many thousands of science professionals ...

  8. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Parallelized Confocal Microscopy (Spinning Disk) increases the speed at which a confocal image can be acquired...utilizes a configuration of two or more objectives to create a thin sheet of excitation light that propagates...molecules on and off. This can provide a 2-10 fold increase in resolution, down to 10s of nanometers. Thick...samples due to its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for...

  9. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...year’s impressive entries, but our community of creative scientists delivered some spectacular photos once...supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament ever! & they...winners. We hope everyone had a blast decorating and creating memories with their labmates! Are you inspired...

  10. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...the world to help increase global access to materials in our repository. This incredible gift will allow...a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos...
Showing: 391 - 400 of 790 results